論文 - 詳細
| RRC ID | 53402 |
|---|---|
| 著者 | Chen H, Li H, Wang D. |
| タイトル | Graphene Oxide Dysregulates Neuroligin/NLG-1-Mediated Molecular Signaling in Interneurons in Caenorhabditis elegans. |
| ジャーナル | Sci Rep |
| Abstract |
Graphene oxide (GO) can be potentially used in many medical and industrial fields. Using assay system of Caenorhabditis elegans, we identified the NLG-1/Neuroligin-mediated neuronal signaling dysregulated by GO exposure. In nematodes, GO exposure significantly decreased the expression of NLG-1, a postsynaptic cell adhesion protein. Loss-of-function mutation of nlg-1 gene resulted in a susceptible property of nematodes to GO toxicity. Rescue experiments suggested that NLG-1 could act in AIY interneurons to regulate the response to GO exposure. In the AIY interneurons, PKC-1, a serine/threonine protein kinase C (PKC) protein, was identified as the downstream target for NLG-1 in the regulation of response to GO exposure. LIN-45, a Raf protein in ERK signaling pathway, was further identified as the downstream target for PKC-1 in the regulation of response to GO exposure. Therefore, GO may dysregulate NLG-1-mediated molecular signaling in the interneurons, and a neuronal signaling cascade of NLG-1-PKC-1-LIN-45 was raised to be required for the control of response to GO exposure. More importantly, intestinal RNAi knockdown of daf-16 gene encoding a FOXO transcriptional factor in insulin signaling pathway suppressed the resistant property of nematodes overexpressing NLG-1 to GO toxicity, suggesting the possible link between neuronal NLG-1 signaling and intestinal insulin signaling in the regulation of response to GO exposure. |
| 巻・号 | 7 |
| ページ | 41655 |
| 公開日 | 2017-1-27 |
| DOI | 10.1038/srep41655 |
| PII | srep41655 |
| PMID | 28128356 |
| PMC | PMC5269675 |
| MeSH | Animals Biological Transport Caenorhabditis elegans Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Cell Adhesion Molecules, Neuronal / genetics* Cell Adhesion Molecules, Neuronal / metabolism* Epistasis, Genetic Forkhead Transcription Factors / genetics Forkhead Transcription Factors / metabolism Gene Expression Regulation / genetics Graphite / chemistry Graphite / pharmacology* Interneurons / drug effects* Interneurons / metabolism* Models, Biological Mutation Organ Specificity / genetics Oxides* / chemistry Protein Kinase C / metabolism Signal Transduction / drug effects* raf Kinases / genetics raf Kinases / metabolism |
| IF | 3.998 |
| 引用数 | 18 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1961 tm494 |