Reference - Detail
| RRC ID | 53458 |
|---|---|
| Author | Chi C, Ronai D, Than MT, Walker CJ, Sewell AK, Han M. |
| Title | Nucleotide levels regulate germline proliferation through modulating GLP-1/Notch signaling in C. elegans. |
| Journal | Genes Dev |
| Abstract |
Animals alter their reproductive programs to accommodate changes in nutrient availability, yet the connections between known nutrient-sensing systems and reproductive programs are underexplored, and whether there is a mechanism that senses nucleotide levels to coordinate germline proliferation is unknown. We established a model system in which nucleotide metabolism is perturbed in both the nematode Caenorhabditis elegans (cytidine deaminases) and its food (Escherichia coli); when fed food with a low uridine/thymidine (U/T) level, germline proliferation is arrested. We provide evidence that this impact of U/T level on the germline is critically mediated by GLP-1/Notch and MPK-1/MAPK, known to regulate germline mitotic proliferation. This germline defect is suppressed by hyperactivation of glp-1 or disruption of genes downstream from glp-1 to promote meiosis but not by activation of the IIS or TORC1 pathways. Moreover, GLP-1 expression is post-transcriptionally modulated by U/T levels. Our results reveal a previously unknown nucleotide-sensing mechanism for controlling reproductivity. |
| Volume | 30(3) |
| Pages | 307-20 |
| Published | 2016-2-1 |
| DOI | 10.1101/gad.275107.115 |
| PII | 30/3/307 |
| PMID | 26833730 |
| PMC | PMC4743060 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Cell Proliferation / genetics Escherichia coli / genetics Fertility / genetics Germ Cells Glucagon-Like Peptide 1 / metabolism* Mutation Nucleotides / metabolism* Receptors, Notch / metabolism* Signal Transduction* |
| IF | 9.527 |
| Times Cited | 21 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 6 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm742 |