RRC ID 53634
著者 Maslowska KH, Makiela-Dzbenska K, Fijalkowska IJ, Schaaper RM.
タイトル Suppression of the E. coli SOS response by dNTP pool changes.
ジャーナル Nucleic Acids Res
Abstract The Escherichia coli SOS system is a well-established model for the cellular response to DNA damage. Control of SOS depends largely on the RecA protein. When RecA is activated by single-stranded DNA in the presence of a nucleotide triphosphate cofactor, it mediates cleavage of the LexA repressor, leading to expression of the 30(+)-member SOS regulon. RecA activation generally requires the introduction of DNA damage. However, certain recA mutants, like recA730, bypass this requirement and display constitutive SOS expression as well as a spontaneous (SOS) mutator effect. Presently, we investigated the possible interaction between SOS and the cellular deoxynucleoside triphosphate (dNTP) pools. We found that dNTP pool changes caused by deficiencies in the ndk or dcd genes, encoding nucleoside diphosphate kinase and dCTP deaminase, respectively, had a strongly suppressive effect on constitutive SOS expression in recA730 strains. The suppression of the recA730 mutator effect was alleviated in a lexA-deficient background. Overall, the findings suggest a model in which the dNTP alterations in the ndk and dcd strains interfere with the activation of RecA, thereby preventing LexA cleavage and SOS induction.
巻・号 43(8)
ページ 4109-20
公開日 2015-4-30
DOI 10.1093/nar/gkv217
PII gkv217
PMID 25824947
PMC PMC4417155
MeSH Bacterial Proteins / genetics DNA-Directed DNA Polymerase / metabolism Deoxyribonucleotides / metabolism* Drug Resistance, Bacterial / genetics Escherichia coli / drug effects* Escherichia coli / genetics Escherichia coli / metabolism Escherichia coli Proteins / metabolism Mutagenesis Mutation Nucleoside-Diphosphate Kinase / genetics Nucleotide Deaminases / genetics Rec A Recombinases / genetics Regulon Rifampin / pharmacology SOS Response, Genetics* Serine Endopeptidases / genetics Suppression, Genetic*
IF 11.502
引用数 9
リソース情報
原核生物(大腸菌)