論文 - 詳細
| RRC ID | 53850 |
|---|---|
| 著者 | Kuse Y, Ohuchi K, Nakamura S, Hara H, Shimazawa M. |
| タイトル | Microglia increases the proliferation of retinal precursor cells during postnatal development. |
| ジャーナル | Mol Vis |
| Abstract |
Purpose:In mice, retinal development continues throughout the postnatal stage accompanied by the proliferation of retinal precursor cells. Previous reports showed that during the postnatal stage microglia increase from postnatal day 0 (P0) to P7. However, how microglia are associated with retinal development remains unknown. Methods:The involvement of microglia in retinal development was investigated by two approaches, microglial activation and loss, using lipopolysaccharide (LPS) and PLX3397 (pexidartinib), respectively. Results:LPS injection at 1 mg/kg, intraperitoneally (i.p.) in the neonatal mice increased the number of retinal microglia at P7. 5-Bromo-2´-deoxyuridine (BrdU)-positive proliferative cells were increased by LPS treatment compared to the control group. The proliferative cells were mainly colocalized with paired box 6 (Pax6), a marker of retinal precursor cells. However, the depletion of microglia by treatment with PLX3397 decreased the BrdU-positive proliferative cells. Moreover, progranulin deficiency decreased the number of microglia and retinal precursor cells. Conclusions:These findings indicated that microglia regulate the proliferation of immature retinal cells. |
| 巻・号 | 24 |
| ページ | 536-545 |
| 公開日 | 2018-7-30 |
| PMID | 30090016 |
| PMC | PMC6066272 |
| MeSH | Aminopyridines / pharmacology Animals Animals, Newborn Bromodeoxyuridine Calbindins / genetics Calbindins / metabolism Calcium-Binding Proteins / genetics Calcium-Binding Proteins / metabolism Cell Communication / drug effects Cell Proliferation / drug effects Gene Expression Regulation, Developmental* Granulins Injections, Intraperitoneal Intercellular Signaling Peptides and Proteins / deficiency Intercellular Signaling Peptides and Proteins / genetics* Lipopolysaccharides / pharmacology Mice Mice, Inbred C57BL Microfilament Proteins / genetics Microfilament Proteins / metabolism Microglia / cytology* Microglia / drug effects Microglia / metabolism Nestin / genetics Nestin / metabolism Organogenesis / drug effects Organogenesis / genetics PAX6 Transcription Factor / genetics* PAX6 Transcription Factor / metabolism Progranulins Pyrroles / pharmacology Retina / cytology* Retina / drug effects Retina / metabolism Rhodopsin / genetics Rhodopsin / metabolism Stem Cells / cytology* Stem Cells / drug effects Stem Cells / metabolism |
| IF | 2.202 |
| 引用数 | 2 |
| リソース情報 | |
| 実験動物マウス | RBRC02370 |