RRC ID 53850
Author Kuse Y, Ohuchi K, Nakamura S, Hara H, Shimazawa M.
Title Microglia increases the proliferation of retinal precursor cells during postnatal development.
Journal Mol Vis
Abstract Purpose:In mice, retinal development continues throughout the postnatal stage accompanied by the proliferation of retinal precursor cells. Previous reports showed that during the postnatal stage microglia increase from postnatal day 0 (P0) to P7. However, how microglia are associated with retinal development remains unknown.
Methods:The involvement of microglia in retinal development was investigated by two approaches, microglial activation and loss, using lipopolysaccharide (LPS) and PLX3397 (pexidartinib), respectively.
Results:LPS injection at 1 mg/kg, intraperitoneally (i.p.) in the neonatal mice increased the number of retinal microglia at P7. 5-Bromo-2´-deoxyuridine (BrdU)-positive proliferative cells were increased by LPS treatment compared to the control group. The proliferative cells were mainly colocalized with paired box 6 (Pax6), a marker of retinal precursor cells. However, the depletion of microglia by treatment with PLX3397 decreased the BrdU-positive proliferative cells. Moreover, progranulin deficiency decreased the number of microglia and retinal precursor cells.
Conclusions:These findings indicated that microglia regulate the proliferation of immature retinal cells.
Volume 24
Pages 536-545
Published 2018-7-30
PMID 30090016
PMC PMC6066272
MeSH Aminopyridines / pharmacology Animals Animals, Newborn Bromodeoxyuridine Calbindins / genetics Calbindins / metabolism Calcium-Binding Proteins / genetics Calcium-Binding Proteins / metabolism Cell Communication / drug effects Cell Proliferation / drug effects Gene Expression Regulation, Developmental* Granulins Injections, Intraperitoneal Intercellular Signaling Peptides and Proteins / deficiency Intercellular Signaling Peptides and Proteins / genetics* Lipopolysaccharides / pharmacology Mice Mice, Inbred C57BL Microfilament Proteins / genetics Microfilament Proteins / metabolism Microglia / cytology* Microglia / drug effects Microglia / metabolism Nestin / genetics Nestin / metabolism Organogenesis / drug effects Organogenesis / genetics PAX6 Transcription Factor / genetics* PAX6 Transcription Factor / metabolism Progranulins Pyrroles / pharmacology Retina / cytology* Retina / drug effects Retina / metabolism Rhodopsin / genetics Rhodopsin / metabolism Stem Cells / cytology* Stem Cells / drug effects Stem Cells / metabolism
IF 2.202
Times Cited 2
Mice RBRC02370