論文 - 詳細
| RRC ID | 53948 |
|---|---|
| 著者 | Reich DP, Tyc KM, Bass BL. |
| タイトル | C. elegans ADARs antagonize silencing of cellular dsRNAs by the antiviral RNAi pathway. |
| ジャーナル | Genes Dev |
| Abstract |
Cellular dsRNAs are edited by adenosine deaminases that act on RNA (ADARs). While editing can alter mRNA-coding potential, most editing occurs in noncoding sequences, the function of which is poorly understood. Using dsRNA immunoprecipitation (dsRIP) and RNA sequencing (RNA-seq), we identified 1523 regions of clustered A-to-I editing, termed editing-enriched regions (EERs), in four stages of Caenorhabditis elegans development, often with highest expression in embryos. Analyses of small RNA-seq data revealed 22- to 23-nucleotide (nt) siRNAs, reminiscent of viral siRNAs, that mapped to EERs and were abundant in adr-1;adr-2 mutant animals. Consistent with roles for these siRNAs in silencing, EER-associated genes (EAGs) were down-regulated in adr-1;adr-2 embryos, and this was dependent on associated EERs and the RNAi factor RDE-4. We observed that ADARs genetically interact with the 26G endogenous siRNA (endo-siRNA) pathway, which likely competes for RNAi components; deletion of factors required for this pathway (rrf-3 or ergo-1) in adr-1;adr-2 mutant strains caused a synthetic phenotype that was rescued by deleting antiviral RNAi factors. Poly(A)+ RNA-seq revealed EAG down-regulation and antiviral gene induction in adr-1;adr-2;rrf-3 embryos, and these expression changes were dependent on rde-1 and rde-4 Our data suggest that ADARs restrict antiviral silencing of cellular dsRNAs. |
| 巻・号 | 32(3-4) |
| ページ | 271-282 |
| 公開日 | 2018-2-1 |
| DOI | 10.1101/gad.310672.117 |
| PII | gad.310672.117 |
| PMID | 29483152 |
| PMC | PMC5859968 |
| MeSH | Adenosine / metabolism Adenosine Deaminase / genetics* Animals Caenorhabditis elegans / embryology Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Inosine / metabolism Mutation RNA Editing* RNA Interference* RNA, Double-Stranded / metabolism* RNA, Small Interfering / metabolism RNA-Dependent RNA Polymerase / genetics Ribonuclease III / metabolism |
| IF | 9.527 |
| 引用数 | 14 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm668 |