論文 - 詳細
| RRC ID | 53959 |
|---|---|
| 著者 | Kisielnicka E, Minasaki R, Eckmann CR. |
| タイトル | MAPK signaling couples SCF-mediated degradation of translational regulators to oocyte meiotic progression. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
RNA-binding proteins (RBPs) are important regulators of gene expression programs, especially during gametogenesis. How the abundance of particular RBPs is restricted to defined stages of meiosis remains largely elusive. Here, we report a molecular pathway that subjects two nonrelated but broadly evolutionarily conserved translational regulators (CPB-3/CPEB and GLD-1/STAR) to proteosomal degradation in Caenorhabditis elegans germ cells at the transition from pachytene to diplotene of meiotic prophase. Both RBPs are recognized by the same ubiquitin ligase complex, containing the molecular scaffold Cullin-1 and the tumor suppressor SEL-10/FBXW7 as its substrate recognition subunit. Destabilization of either RBP through this Skp, Cullin, F-box-containing complex (SCF) ubiquitin ligase appears to loosen its negative control over established target mRNAs, and presumably depends on a prior phosphorylation of CPB-3 and GLD-1 by MAPK (MPK-1), whose activity increases in mid- to late pachytene to promote meiotic progression and oocyte differentiation. Thus, we propose that the orchestrated degradation of RBPs via MAPK-signaling cascades during germ cell development may act to synchronize meiotic with sexual differentiation gene expression changes. |
| 巻・号 | 115(12) |
| ページ | E2772-E2781 |
| 公開日 | 2018-3-20 |
| DOI | 10.1073/pnas.1715439115 |
| PII | 1715439115 |
| PMID | 29496961 |
| PMC | PMC5866554 |
| MeSH | Animals Animals, Genetically Modified Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism MAP Kinase Signaling System Meiosis / physiology* Mitogen-Activated Protein Kinase 1 / genetics Mitogen-Activated Protein Kinase 1 / metabolism Oocytes / metabolism Oocytes / physiology* Oogenesis / physiology Phosphorylation Protein Processing, Post-Translational RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism* Ubiquitin-Protein Ligases / metabolism Ubiquitination |
| IF | 9.412 |
| 引用数 | 5 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 12 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1746 |