RRC ID 53965
Author Seth M, Shirayama M, Tang W, Shen EZ, Tu S, Lee HC, Weng Z, Mello CC.
Title The Coding Regions of Germline mRNAs Confer Sensitivity to Argonaute Regulation in C. elegans.
Journal Cell Rep
Abstract Protein-coding genes undergo a wide array of regulatory interactions with factors that engage non-coding regions. Open reading frames (ORFs), in contrast, are thought to be constrained by coding function, precluding a major role in gene regulation. Here, we explore Piwi-interacting (pi)RNA-mediated transgene silencing in C. elegans and show that marked differences in the sensitivity to piRNA silencing map to the endogenous sequences within transgene ORFs. Artificially increasing piRNA targeting within the ORF of a resistant transgene can lead to a partial yet stable reduction in expression, revealing that piRNAs not only silence but can also "tune" gene expression. Our findings support a model that involves a temporal element to mRNA regulation by germline Argonautes, likely prior to translation, and suggest that piRNAs afford incremental control of germline mRNA expression by targeting the body of the mRNA, including the coding region.
Volume 22(9)
Pages 2254-2264
Published 2018-2-27
DOI 10.1016/j.celrep.2018.02.009
PII S2211-1247(18)30177-3
PMID 29456100
PMC PMC5918280
MeSH Animals Argonaute Proteins / genetics* Argonaute Proteins / metabolism Base Sequence Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Codon, Nonsense / genetics Gene Expression Regulation* Gene Silencing Germ Cells / metabolism* Models, Biological Open Reading Frames / genetics* RNA, Messenger / genetics RNA, Messenger / metabolism RNA, Small Interfering / metabolism Signal Transduction Transgenes
IF 7.815
Times Cited 15
Resource
C.elegans tm872