| RRC ID |
53965
|
| 著者 |
Seth M, Shirayama M, Tang W, Shen EZ, Tu S, Lee HC, Weng Z, Mello CC.
|
| タイトル |
The Coding Regions of Germline mRNAs Confer Sensitivity to Argonaute Regulation in C. elegans.
|
| ジャーナル |
Cell Rep
|
| Abstract |
Protein-coding genes undergo a wide array of regulatory interactions with factors that engage non-coding regions. Open reading frames (ORFs), in contrast, are thought to be constrained by coding function, precluding a major role in gene regulation. Here, we explore Piwi-interacting (pi)RNA-mediated transgene silencing in C. elegans and show that marked differences in the sensitivity to piRNA silencing map to the endogenous sequences within transgene ORFs. Artificially increasing piRNA targeting within the ORF of a resistant transgene can lead to a partial yet stable reduction in expression, revealing that piRNAs not only silence but can also "tune" gene expression. Our findings support a model that involves a temporal element to mRNA regulation by germline Argonautes, likely prior to translation, and suggest that piRNAs afford incremental control of germline mRNA expression by targeting the body of the mRNA, including the coding region.
|
| 巻・号 |
22(9)
|
| ページ |
2254-2264
|
| 公開日 |
2018-2-27
|
| DOI |
10.1016/j.celrep.2018.02.009
|
| PII |
S2211-1247(18)30177-3
|
| PMID |
29456100
|
| PMC |
PMC5918280
|
| MeSH |
Animals
Argonaute Proteins / genetics*
Argonaute Proteins / metabolism
Base Sequence
Caenorhabditis elegans / genetics*
Caenorhabditis elegans Proteins / genetics*
Caenorhabditis elegans Proteins / metabolism
Codon, Nonsense / genetics
Gene Expression Regulation*
Gene Silencing
Germ Cells / metabolism*
Models, Biological
Open Reading Frames / genetics*
RNA, Messenger / genetics
RNA, Messenger / metabolism
RNA, Small Interfering / metabolism
Signal Transduction
Transgenes
|
| IF |
8.109
|
| 引用数 |
15
|
| リソース情報 |
| 線虫 |
tm872 |
