論文 - 詳細
| RRC ID | 540 |
|---|---|
| 著者 | Funaguma S, Suzuki MG, Tamura T, Shimada T. |
| タイトル | The Bmdsx transgene including trimmed introns is sex-specifically spliced in tissues of the silkworm, Bombyx mori. |
| ジャーナル | J Insect Sci |
| Abstract |
Bmdsx is an orthologue of the sex-determining gene doublesex (dsx) and known to be sex-specifically expressed in various tissues of the silkworm, Bombyx mori. Its pre-mRNA is sex-specifically spliced and encodes female-specific or male-specific polypeptides. The open reading frame of Bmdsx consists of 5 exons, of which exons 3 and 4 are female-specific and its pre-mRNA was known to undergo default processing to generate the female-type mRNA. Previous reports have shown that the mechanism of splicing of the doublesex gene is different in Drosophila melanogaster and Bombyx mori. However, intron 4 is so long that it is difficult to identify the intronic cis-element(s) required for male-specific splicing of Bmdsx pre-mRNA using Bmdsx minigenes whose introns are shortened in various manners. As a first step toward discovery of the cis-element, the Bmdsx mini gene, which consisted of exon 1 and 5 and internally shortened introns 2 to 4, was constructed, and transgenic silkworms expressing this construct were generated. Bmdsx pre-mRNA transcribed derived from transgene was sex-specifically spliced. This result shows that the mini gene contained the information necessary for the correct regulation of alternative splicing. |
| 巻・号 | 5 |
| ページ | 17 |
| 公開日 | 2005-1-1 |
| DOI | 10.1093/jis/5.1.17 |
| PMID | 16341249 |
| PMC | PMC1307578 |
| MeSH | Alternative Splicing* / genetics Animals Animals, Genetically Modified / genetics Animals, Genetically Modified / physiology Bombyx / genetics* Bombyx / metabolism* DNA Primers / chemistry DNA-Binding Proteins / genetics* DNA-Binding Proteins / metabolism* Female Gene Expression Gene Expression Profiling Gene Order / genetics Genetic Vectors / chemical synthesis Genetic Vectors / genetics Insect Proteins / genetics* Insect Proteins / metabolism* Introns / genetics Male Microinjections / methods Polypyrimidine Tract-Binding Protein / genetics Reverse Transcriptase Polymerase Chain Reaction / methods Sex Factors Transformation, Genetic Transgenes / genetics Transgenes / physiology |
| IF | 1.325 |
| 引用数 | 8 |
| WOS 分野 | ENTOMOLOGY |
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| 各媒体での言及数の合計 | 0 |
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