RRC ID 54188
Author Mioka T, Fujimura-Kamada K, Mizugaki N, Kishimoto T, Sano T, Nunome H, Williams DE, Andersen RJ, Tanaka K.
Title Phospholipid flippases and Sfk1p, a novel regulator of phospholipid asymmetry, contribute to low permeability of the plasma membrane.
Journal Mol Biol Cell
Abstract Phospholipid flippase (type 4 P-type ATPase) plays a major role in the generation of phospholipid asymmetry in eukaryotic cell membranes. Loss of Lem3p-Dnf1/2p flippases leads to the exposure of phosphatidylserine (PS) and phosphatidylethanolamine (PE) on the cell surface in yeast, resulting in sensitivity to PS- or PE-binding peptides. We isolated Sfk1p, a conserved membrane protein in the TMEM150/FRAG1/DRAM family, as a multicopy suppressor of this sensitivity. Overexpression of SFK1 decreased PS/PE exposure in lem3Δ mutant cells. Consistent with this, lem3Δ sfk1Δ double mutant cells exposed more PS/PE than the lem3Δ mutant. Sfk1p was previously implicated in the regulation of the phosphatidylinositol-4 kinase Stt4p, but the effect of Sfk1p on PS/PE exposure in lem3Δ was independent of Stt4p. Surprisingly, Sfk1p did not facilitate phospholipid flipping but instead repressed it, even under ATP-depleted conditions. We propose that Sfk1p negatively regulates transbilayer movement of phospholipids irrespective of directions. In addition, we showed that the permeability of the plasma membrane was dramatically elevated in the lem3Δ sfk1Δ double mutant in comparison with the corresponding single mutants. Interestingly, total ergosterol was decreased in the lem3Δ sfk1Δ mutant. Our results suggest that phospholipid asymmetry is required for the maintenance of low plasma membrane permeability.
Volume 29(10)
Pages 1203-1218
Published 2018-5-15
DOI 10.1091/mbc.E17-04-0217
PII mbc.E17-04-0217
PMID 29540528
PMC PMC5935070
MeSH Amino Acid Sequence Cell Membrane / metabolism* Cell Membrane Permeability* Depsipeptides / pharmacology Ergosterol / pharmacology Lipid Bilayers / metabolism Membrane Fluidity / drug effects Membrane Proteins / chemistry Membrane Proteins / metabolism* Models, Biological Mutation / genetics Phosphatidylethanolamines / metabolism Phosphatidylinositols / metabolism Phosphatidylserines / metabolism Phospholipid Transfer Proteins / chemistry Phospholipid Transfer Proteins / metabolism* Phospholipids / metabolism* Saccharomyces cerevisiae / metabolism* Saccharomyces cerevisiae Proteins / chemistry Saccharomyces cerevisiae Proteins / metabolism* Stress, Physiological / drug effects Up-Regulation / drug effects
IF 3.791
Times Cited 9
Resource
DNA material YEp24-SFK1 (RDB16713) YEplac195-SF1 (RDB16714) YEplac195-SFK1 delta C (RDB16715)