RRC ID 54193
Author Hashiguchi M, Tanaka H, Muguerza M, Akashi R, Sandal NN, Andersen SU, Sato S.
Title Lotus japonicus Genetic, Mutant, and Germplasm Resources.
Journal Curr Protoc Plant Biol
Abstract A quarter of a century has passed since Lotus japonicus was proposed as a model legume because of its suitability for molecular genetic studies. Since then, a comprehensive set of genetic resources and tools has been developed, including recombinant inbred lines, a collection of wild accessions, published mutant lines, a large collection of mutant lines tagged with LORE1 insertions, cDNA clones with expressed sequence tag (EST) information, genomic clones with end‐sequence information, and a reference genome sequence. Resource centers in Japan and Denmark ensure easy access to data and materials, and the resources have greatly facilitated L. japonicus research, thereby contributing to the molecular understanding of characteristic legume features such as endosymbiosis. Here, we provide detailed instructions for L. japonicus cultivation and describe how to order materials and access data using the resource center websites. The comprehensive overview presented here will make L. japonicus more easily accessible as a model system, especially for research groups new to L. japonicus research.
Volume 3(2)
Pages e20070
Published 2018-6-19
DOI 10.1002/cppb.20070
PMID 29927119
MeSH Gene Library Genome, Plant Internet Lotus / genetics Lotus / growth & development* Mutation Seed Bank* Seeds
Lotus / Glycine L. japonicus