RRC ID 54330
著者 Tanaka J, Ogawa M, Hojo H, Kawashima Y, Mabuchi Y, Hata K, Nakamura S, Yasuhara R, Takamatsu K, Irié T, Fukada T, Sakai T, Inoue T, Nishimura R, Ohara O, Saito I, Ohba S, Tsuji T, Mishima K.
タイトル Generation of orthotopically functional salivary gland from embryonic stem cells.
ジャーナル Nat Commun
Abstract Organoids generated from pluripotent stem cells are used in the development of organ replacement regenerative therapy by recapitulating the process of organogenesis. These processes are strictly regulated by morphogen signalling and transcriptional networks. However, the precise transcription factors involved in the organogenesis of exocrine glands, including salivary glands, remain unknown. Here, we identify a specific combination of two transcription factors (Sox9 and Foxc1) responsible for the differentiation of mouse embryonic stem cell-derived oral ectoderm into the salivary gland rudiment in an organoid culture system. Following orthotopic transplantation into mice whose salivary glands had been removed, the induced salivary gland rudiment not only showed a similar morphology and gene expression profile to those of the embryonic salivary gland rudiment of normal mice but also exhibited characteristics of mature salivary glands, including saliva secretion. This study suggests that exocrine glands can be induced from pluripotent stem cells for organ replacement regenerative therapy.
巻・号 9(1)
ページ 4216
公開日 2018-10-11
DOI 10.1038/s41467-018-06469-7
PII 10.1038/s41467-018-06469-7
PMID 30310071
PMC PMC6181987
MeSH Animals Cells, Cultured Ectoderm / metabolism Female Gene Expression Profiling HEK293 Cells Humans Mice Mice, Inbred C57BL Mouse Embryonic Stem Cells / cytology* Mouse Embryonic Stem Cells / metabolism Mouth Mucosa / embryology Mouth Mucosa / metabolism Salivary Glands / cytology Salivary Glands / growth & development* Salivary Glands / transplantation Salivary Glands / ultrastructure Transcription Factors / metabolism
IF 12.121
引用数 16
リソース情報
遺伝子材料 Ad-β-gal
ヒト・動物細胞 G4-2(AES0150) EB5(AES0151)