| RRC ID |
54340
|
| Author |
Kobayashi M, Hoshino S, Abe T, Okita N, Tagawa R, Nagai W, Konno R, Suzuki Y, Furuya K, Ishikawa N, Okado H, Oku M, Iwamoto M, Miura Y, Sudo Y, Higami Y.
|
| Title |
Identification of WWP1 as an obesity-associated E3 ubiquitin ligase with a protective role against oxidative stress in adipocytes.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
White adipose tissue (WAT) is not only the main tissue for energy storage but also an endocrine organ that secretes adipokines. Obesity is the most common metabolic disorder and is related to alterations in WAT characteristics, such as chronic inflammation and increasing oxidative stress. WW domain containing E3 ubiquitin protein ligase 1 (WWP1) is a HECT-type ubiquitin E3 ligase that has been implicated in various pathologies. In the present study, we found that WWP1 was upregulated in obese WAT in a p53-dependent manner. To investigate the functions of WWP1 in adipocytes, a proteome analysis of WWP1 overexpression (OE) and knockdown (KD) 3T3-L1 cells was performed. This analysis showed a positive correlation between WWP1 expression and the abundance of several antioxidative proteins. Thus, we measured reactive oxygen species (ROS) in WWP1 OE and KD cells. Consistent with the proteome results, WWP1 OE reduced ROS levels, whereas KD increased them. These findings indicate that WWP1 is an obesity-inducible E3 ubiquitin ligase that can protect against obesity-associated oxidative stress in WAT.
|
| Volume |
508(1)
|
| Pages |
117-122
|
| Published |
2019-1-1
|
| DOI |
10.1016/j.bbrc.2018.11.127
|
| PII |
S0006-291X(18)32560-9
|
| PMID |
30471861
|
| MeSH |
3T3-L1 Cells
Adipocytes / metabolism*
Adipose Tissue, White / metabolism
Animals
Diet, High-Fat / adverse effects
Disease Models, Animal
Gene Knockdown Techniques
Genes, p53
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Obesity / etiology
Obesity / genetics
Obesity / metabolism
Oxidative Stress
Proteome / genetics
Proteome / metabolism
RNA, Messenger / genetics
RNA, Messenger / metabolism
Ubiquitin-Protein Ligases / antagonists & inhibitors
Ubiquitin-Protein Ligases / genetics
Ubiquitin-Protein Ligases / metabolism*
Up-Regulation
|
| IF |
2.985
|
| Times Cited |
1
|
| Resource |
| Mice |
RBRC01361 |
