論文 - 詳細
| RRC ID | 54460 |
|---|---|
| 著者 | Aly H, Akagi K, Ueda H. |
| タイトル | Proteasome activity determines pupation timing through the degradation speed of timer molecule Blimp-1. |
| ジャーナル | Dev Growth Differ |
| Abstract |
The transcriptional repressor Blimp-1 is a labile protein. This characteristic is key for determining pupation timing because the timing of the disappearance of Blimp-1 affects pupation timing by regulating the expression of its target βftz-f1. However, the molecular mechanisms that regulate the protein turnover of Blimp-1 are still unclear. Here, we demonstrate that Blimp-1 is regulated by the ubiquitin proteasome system. We show that Blimp-1 degradation is inhibited by proteasome inhibitor MG132. Pupation timing was delayed in mutants of 26S proteasome subunits as well as FBXO11, which recruits target proteins to the 26S proteasome as a component of the SCF ubiquitin ligase complex by slowing down the degradation speed of Blimp-1. Delay in pupation timing in the FBXO11 mutant was suppressed by the induction of βFTZ-F1. Furthermore, fat-body-specific knockdown of proteasomal activity was sufficient to induce a delay in pupation timing. These results suggest that Blimp-1 is degraded by the 26S proteasome and is recruited by FBXO11 in the fat body, which is important for determining pupation timing. |
| 巻・号 | 60(8) |
| ページ | 502-508 |
| 公開日 | 2018-10-1 |
| DOI | 10.1111/dgd.12569 |
| PMID | 30368781 |
| MeSH | Animals Drosophila Drosophila Proteins / antagonists & inhibitors Drosophila Proteins / metabolism* Enzyme Inhibitors / chemistry Enzyme Inhibitors / pharmacology F-Box Proteins / metabolism Fat Body / metabolism Leupeptins / chemistry Leupeptins / pharmacology Proteasome Endopeptidase Complex / metabolism* Pupa / enzymology Pupa / growth & development* Pupa / metabolism* Repressor Proteins / antagonists & inhibitors Repressor Proteins / metabolism* Time Factors |
| IF | 1.638 |
| 引用数 | 3 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | 4097R–1 4097R-2 |