論文 - 詳細
| RRC ID | 54581 |
|---|---|
| 著者 | Schubert R, Dobritzsch S, Gruber C, Hause G, Athmer B, Schreiber T, Marillonnet S, Okabe Y, Ezura H, Acosta IF, Tarkowska D, Hause B. |
| タイトル | Tomato MYB21 Acts in Ovules to Mediate Jasmonate-Regulated Fertility. |
| ジャーナル | Plant Cell |
| Abstract |
The function of the plant hormone jasmonic acid (JA) in the development of tomato (Solanum lycopersicum) flowers was analyzed with a mutant defective in JA perception (jasmonate-insensitive1-1, jai1-1). In contrast with Arabidopsis (Arabidopsis thaliana) JA-insensitive plants, which are male sterile, the tomato jai1-1 mutant is female sterile, with major defects in female development. To identify putative JA-dependent regulatory components, we performed transcriptomics on ovules from flowers at three developmental stages from wild type and jai1-1 mutants. One of the strongly downregulated genes in jai1-1 encodes the MYB transcription factor SlMYB21. Its Arabidopsis ortholog plays a crucial role in JA-regulated stamen development. SlMYB21 was shown here to exhibit transcription factor activity in yeast, to interact with SlJAZ9 in yeast and in planta, and to complement Arabidopsis myb21-5 To analyze SlMYB21 function, we generated clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR associated protein 9 (Cas9) mutants and identified a mutant by Targeting Induced Local Lesions in Genomes (TILLING). These mutants showed female sterility, corroborating a function of MYB21 in tomato ovule development. Transcriptomics analysis of wild type, jai1-1, and myb21-2 carpels revealed processes that might be controlled by SlMYB21. The data suggest positive regulation of JA biosynthesis by SlMYB21, but negative regulation of auxin and gibberellins. The results demonstrate that SlMYB21 mediates at least partially the action of JA and might control the flower-to-fruit transition.
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| 巻・号 | 31(5) |
| ページ | 1043-1062 |
| 公開日 | 2019-5-1 |
| DOI | 10.1105/tpc.18.00978 |
| PII | tpc.18.00978 |
| PMID | 30894458 |
| PMC | PMC6533027 |
| MeSH | Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Cyclopentanes / metabolism* Down-Regulation Fertility Flowers / genetics Flowers / physiology Fruit / genetics Fruit / physiology Gene Expression Regulation, Plant* Gibberellins / metabolism Indoleacetic Acids / metabolism Mutation Ovule / genetics Ovule / physiology Oxylipins / metabolism* Phenotype Plant Growth Regulators / metabolism* Plant Infertility Plant Proteins / genetics Plant Proteins / metabolism* Solanum lycopersicum / genetics* Solanum lycopersicum / physiology Transcription Factors / genetics Transcription Factors / metabolism* |
| IF | 8.631 |
| 引用数 | 10 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 48 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| トマト | TOMJPF00001 TOMJPE7564 TOMJPE7979 TOMJPE8245 TOMJPW0469 |