RRC ID 54609
Author Rajniak J, Giehl RFH, Chang E, Murgia I, von Wirén N, Sattely ES.
Title Biosynthesis of redox-active metabolites in response to iron deficiency in plants.
Journal Nat Chem Biol
Abstract Iron is an essential but poorly bioavailable nutrient because of its low solubility, especially in alkaline soils. Here, we describe the discovery of a previously undescribed redox-active catecholic metabolite, termed sideretin, which derives from the coumarin fraxetin and is the primary molecule exuded by Arabidopsis thaliana roots in response to iron deficiency. We identified two enzymes that complete the biosynthetic pathway of fraxetin and sideretin. Chemical characterization of fraxetin and sideretin, and biological assays with pathway mutants, suggest that these coumarins are critical for iron nutrition in A. thaliana. Further, we show that sideretin production also occurs in eudicot species only distantly related to A. thaliana. Untargeted metabolomics of the root exudates of various eudicots revealed production of structurally diverse redox-active molecules in response to iron deficiency. Our results indicate that secretion of small-molecule reductants by roots may be a widespread and previously underappreciated component of reduction-based iron uptake.
Volume 14(5)
Pages 442-450
Published 2018-5-1
DOI 10.1038/s41589-018-0019-2
PII 10.1038/s41589-018-0019-2
PMID 29581584
PMC PMC6693505
MeSH Arabidopsis / metabolism* Arabidopsis Proteins / metabolism Coumarins / metabolism* Gene Expression Regulation, Plant Genetic Complementation Test Iron / deficiency* Kinetics Metabolomics Mutation Oxidation-Reduction* Phenotype Phylogeny Plant Roots / metabolism* Plants, Genetically Modified Rhizosphere Scopoletin / metabolism Solubility Thermodynamics
IF 12.154
Times Cited 51
Resource
Arabidopsis / Cultured plant cells, genes pda08472