RRC ID 54681
著者 Matsuo J, Haga S, Hashimoto K, Okubo T, Ozawa T, Ozaki M, Yamaguchi H.
タイトル Activation of caspase-3 during Chlamydia trachomatis-induced apoptosis at a late stage.
ジャーナル Can J Microbiol
Abstract The obligate intracellular bacterium Chlamydia trachomatis activates the host cell apoptosis pathway at a late stage of its developmental cycle. However, whether caspase-3, which is a key enzyme of apoptosis, is activated in Chlamydia-infected cells remains unknown. Here, we established HEp-2 cells stably expressing cFluc-DEVD, which is a caspase-3 substrate sequence inserted into cyclic firefly luciferase, and then monitored the dynamics of caspase-3 activity in cells infected with Chlamydia. Transfected cells without infection showed a significant increase in luciferase activity due to stimulation with staurosporine, an inducer of apoptosis. Activation was significantly blocked by addition of caspase inhibitor z-VAD-fmk. Furthermore, as expected, Chlamydia infection caused a significant increase in luciferase activation at 36-48 h postinfection with a contrastive decrease at 24 h postinfection, which is already well known. Such activation caused by the infection was much stronger when the amount of bacteria was increased. Thus, caspase-3 activation was accurately monitored by the luciferase activity in HEp-2 cells constitutively expressing the cFluc-DEVD probe. Furthermore, our data showed that C. trachomatis activates caspase-3 in host cells at a late stage of infection.
巻・号 65(2)
ページ 135-143
公開日 2019-2-1
DOI 10.1139/cjm-2018-0408
PMID 30336068
MeSH Apoptosis* Caspase 3 / metabolism* Cells, Cultured Chlamydia trachomatis / physiology* Enzyme Activation Humans
IF 1.55
引用数 2
リソース情報
ヒト・動物細胞 HEp-2(RCB1889) HeLa