RRC ID 54690
Author Sato T, Ogawa T.
Title Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated Method.
Journal Methods Mol Biol
Abstract Mouse spermatogonial stem cells (SSCs) can be grown in culture for long periods. Cultured SSCs, also called germline stem (GS) cells, maintain themselves by self-renewing proliferation while retaining the ability to differentiate into sperm. Thus, when transplanted into the seminiferous tubules of a host mouse testis, they settle in the basal compartment of the tubules and establish spermatogenenic colonies. The sperm produced in the host are competent to produce offspring. This can be exploited for the generation of genetically modified mice, through the transplantation of genetically modified GS cells. In this section, we describe a method of genome editing-mediated GS cell modification and transplantation.
Volume 1874
Pages 87-98
Published 2019-1-1
DOI 10.1007/978-1-4939-8831-0_5
PMID 30353509
MeSH Adult Germline Stem Cells / chemistry Adult Germline Stem Cells / cytology* Animals Cell Culture Techniques Cell Differentiation Cell Proliferation Cells, Cultured Female Gene Editing / methods* Gene Knock-In Techniques Male Mice Mice, Transgenic / genetics Mice, Transgenic / growth & development* RNA, Untranslated / genetics* Rats Spermatogenesis Stem Cell Transplantation
Human and Animal Cells GS-DG1(AES0002) Mvh-Venus GS(AES0196)