RRC ID 54793
Author Yasuda H, Sonoda A, Yamamoto M, Kawashima Y, Takishita Y, Morita A, Tsutsumi T, Tsuchiya M, Sato EF.
Title 17-β-estradiol enhances neutrophil extracellular trap formation by interaction with estrogen membrane receptor.
Journal Arch Biochem Biophys
Abstract Cell death-associated neutrophil extracellular trap formation (NETosis) occurs during various autoimmune diseases including systemic lupus erythematosus and rheumatoid arthritis, as well as during gestation. Although increasing estrogen concentrations associated with pregnancy might induce NETosis via nuclear estrogen receptor (ERα/ERβ), little is known about the mechanisms associated with estrogen-induced NETosis. Here, we investigated the effects of estrogen (17-β-estradiol; E2) on NETosis, focusing on mechanisms associated with estrogen membrane receptor (GPR30) in neutrophil-like HL-60 cells. Our results show that E2 and the GPR30 agonist G-1 increases level of NETosis and NET formation. Moreover, NETosis-associated intracellular and extracellular histone citrullination and peptidyl arginine deiminase 4 (PAD4) expression were also increased by E2 or G-1 treatment. Furthermore, GPR30 antagonist pre-treatment inhibited increases in NETosis and PAD4 expression mediated by G-1 and partially inhibited these effects mediated by E2. These results demonstrate that E2 treatment induces NETosis via not only ERα/ERβ but also GPR30 in neutrophil-like HL-60 cells.
Volume 663
Pages 64-70
Published 2019-3-15
DOI 10.1016/j.abb.2018.12.028
PII S0003-9861(18)30803-8
PMID 30590021
MeSH Calcimycin / metabolism Cell Differentiation / drug effects Cell Membrane / metabolism Dimethyl Sulfoxide / pharmacology Estradiol / metabolism Estradiol / pharmacology* Extracellular Traps / drug effects* HL-60 Cells Histones / metabolism Humans NADPH Oxidases / metabolism Neutrophils / cytology Neutrophils / metabolism Protein Binding Protein-Arginine Deiminase Type 4 / metabolism Reactive Oxygen Species / metabolism Receptors, Estrogen / metabolism* Receptors, G-Protein-Coupled / agonists
IF 3.559
Times Cited 1
Resource
Human and Animal Cells HL-60(RCB3683)