論文 - 詳細
| RRC ID | 54849 |
|---|---|
| 著者 | Zhang W, Takahara T, Achiha T, Shibata H, Maki M. |
| タイトル | Cellular Ca2+-Responding Nanoluciferase Reporter Gene System Directed by Tandemly Repeated Pseudo-palindromic NFAT-Response Elements. |
| ジャーナル | Methods Mol Biol |
| Abstract |
Luciferase reporter gene systems based on the NFAT-response element (RE) have been used to monitor intracellular Ca2+ elevation. However, Ca2+ mobilization agent (e.g., ionomycin) alone is not adequate to activate the currently often employed reporter gene that contains the NFAT-RE found in the IL2 promoter. In addition to activation of NFAT through the Ca2+-calmodulin/calcineurin pathway, activation of AP-1 as a partner transcription factor is essential for the IL2-based NFAT-RE system. Here, we describe a detailed method for the recently developed new reporter gene system containing the NFAT-RE from the IL8 promoter. This system enables us to monitor endpoint effects of Ca2+-mobilizing agonists independent of AP-1 activation. |
| 巻・号 | 1929 |
| ページ | 95-109 |
| 公開日 | 2019-1-1 |
| DOI | 10.1007/978-1-4939-9030-6_7 |
| PMID | 30710269 |
| MeSH | Calcineurin / genetics Calcium / analysis* Calmodulin / genetics Gene Expression Regulation Genes, Reporter* HEK293 Cells Humans Interleukin-2 / chemistry Interleukin-2 / genetics* NFATC Transcription Factors / metabolism Promoter Regions, Genetic Transcription Factor AP-1 / genetics Transcriptional Activation |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 293(RCB1637) |