| RRC ID |
54849
|
| 著者 |
Zhang W, Takahara T, Achiha T, Shibata H, Maki M.
|
| タイトル |
Cellular Ca2+-Responding Nanoluciferase Reporter Gene System Directed by Tandemly Repeated Pseudo-palindromic NFAT-Response Elements.
|
| ジャーナル |
Methods Mol Biol
|
| Abstract |
Luciferase reporter gene systems based on the NFAT-response element (RE) have been used to monitor intracellular Ca2+ elevation. However, Ca2+ mobilization agent (e.g., ionomycin) alone is not adequate to activate the currently often employed reporter gene that contains the NFAT-RE found in the IL2 promoter. In addition to activation of NFAT through the Ca2+-calmodulin/calcineurin pathway, activation of AP-1 as a partner transcription factor is essential for the IL2-based NFAT-RE system. Here, we describe a detailed method for the recently developed new reporter gene system containing the NFAT-RE from the IL8 promoter. This system enables us to monitor endpoint effects of Ca2+-mobilizing agonists independent of AP-1 activation.
|
| 巻・号 |
1929
|
| ページ |
95-109
|
| 公開日 |
2019-1-1
|
| DOI |
10.1007/978-1-4939-9030-6_7
|
| PMID |
30710269
|
| MeSH |
Calcineurin / genetics
Calcium / analysis*
Calmodulin / genetics
Gene Expression Regulation
Genes, Reporter*
HEK293 Cells
Humans
Interleukin-2 / chemistry
Interleukin-2 / genetics*
NFATC Transcription Factors / metabolism
Promoter Regions, Genetic
Transcription Factor AP-1 / genetics
Transcriptional Activation
|
| リソース情報 |
| ヒト・動物細胞 |
293(RCB1637) |
