RRC ID 55038
Author Lü J, Izvolsky KI, Qian J, Cardoso WV.
Title Identification of FGF10 targets in the embryonic lung epithelium during bud morphogenesis.
Journal J. Biol. Chem.
Abstract Genetic studies implicate Fgf10-Fgfr2 signaling as a critical regulator of bud morphogenesis in the embryo. However, little is known about the transcriptional targets of Fgf10 during this process. Here we identified global changes in gene expression in lung epithelial explants undergoing FGF10-mediated budding in the absence of other growth factors and mesenchyme. Targets were confirmed by their localization at sites where endogenous Fgf10 signaling is active in embryonic lungs and by demonstrating their induction in intact lungs in response to local application of FGF10 protein. We show that the initial stages of budding are characterized by marked up-regulation of genes associated with cell rearrangement and cell migration, inflammatory process, and lipid metabolism but not cell proliferation. We also found that some genes implicated in tumor invasion and metastatic behavior are epithelial targets of Fgf10 in the lung and other developing organs that depend on Fgf10-Fgfr2 signaling to properly form. Our approach identifies Fgf10 targets that are common to multiple biological processes and provides insights into potential mechanisms by which Fgf signaling regulates epithelial cell behavior.
Volume 280(6)
Pages 4834-41
Published 2005-2-11
DOI 10.1074/jbc.M410714200
PII M410714200
PMID 15556938
MeSH Animals Cell Adhesion Cell Movement Cell Proliferation DNA Primers / chemistry Embryo, Mammalian / metabolism* Epithelium / metabolism Fibroblast Growth Factor 10 Fibroblast Growth Factors / metabolism Fibroblast Growth Factors / physiology* Gene Expression Regulation, Developmental In Situ Hybridization Inflammation Lung / embryology* Mesoderm / metabolism Mice Oligonucleotide Array Sequence Analysis RNA / metabolism Receptor Protein-Tyrosine Kinases / metabolism Receptor, Fibroblast Growth Factor, Type 2 Receptors, Fibroblast Growth Factor / metabolism Signal Transduction Time Factors Transcription, Genetic Up-Regulation
IF 4.011
Resource
DNA material NIA 15K Mouse cDNA Clone H3029F01 (MCH011721) H3031E05 (MCH012501) H3034H10 (MCH013778) H3040F10 (MCH016130) H3047A09 (MCH018809) H3059F12 (MCH023732) H3131D12 (MCH052484) H3146E07 (MCH058503)