論文 - 詳細
| RRC ID | 55207 |
|---|---|
| 著者 | Hirota K, Nishita M, Tu Z, Matsuyama H, Yumoto I. |
| タイトル | Bacillus fermenti sp. nov., an indigo-reducing obligate alkaliphile isolated from indigo fermentation liquor for dyeing. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
The indigo-reducing, facultatively anaerobic and obligately alkaliphilic strains Bf-1T, Bf-2 and Bf-4 were isolated from an indigo fermentation liquor used for dyeing, which uses sukumo [composted Polygonum indigo (Polygonum tinctorium Lour.) leaves] as a basic ingredient and was obtained from a craft centre in Date City, Hokkaido, Japan. The 16S rRNA gene sequence analyses indicated that the closest neighbours of strain Bf-1T are Bacillus maritimus DSM 100413T (98.3 % 16S rRNA gene sequence similarity), Bacillus persicus DSM 25386T (98.2 %) and Bacillus rigiliprofundi LMG 28275T (97.7 %). The 16S rRNA gene sequence of strain Bf-1T was almost identical to the sequences of strains Bf-2 and Bf-4 (99.9 %). Cells of strain Bf-1T stained Gram-positive and formed straight rods that achieved motility through a pair of subpolar flagella. Strain Bf-1T grew at temperatures of between 15 and 45 °C with optimum growth at 33‒40 °C. The strain grew in the pH range of pH 8‒12, with optimum growth at pH 10. The isoprenoid quinone detected was menaquinone-7 (MK-7), and the DNA G+C content was 41.7 %. The whole-cell fatty acid profile mainly (>10 %) consisted of iso-C15 : 0 and iso-C16 : 0. Phylogenetically related neighbours, although demonstrating high 16S rRNA gene sequence similarity (>97.6 %) with strain Bf-1T, exhibited less than 9 % relatedness in DNA-DNA hybridization experiments. Based on evidence from this polyphasic study, the isolates represent a novel species, for which the name Bacillus fermenti sp. nov. is proposed. The type strain of this species is Bf-1T (=JCM 31807T=NCIMB 15079T). |
| 巻・号 | 68(4) |
| ページ | 1123-1129 |
| 公開日 | 2018-4-1 |
| DOI | 10.1099/ijsem.0.002636 |
| PMID | 29458563 |
| MeSH | Bacillus / classification* Bacillus / genetics Bacillus / isolation & purification Bacterial Typing Techniques Base Composition Coloring Agents* DNA, Bacterial / genetics Fatty Acids / chemistry Fermentation* Indigo Carmine / analysis Japan Nucleic Acid Hybridization Phylogeny* Polygonum / microbiology* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Vitamin K 2 / analogs & derivatives Vitamin K 2 / chemistry |
| IF | 2.166 |
| 引用数 | 4 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 31807 |