RRC ID 55528
著者 Zhou EM, Xian WD, Jiao JY, Liu L, Li MM, Ding YP, Yin YR, Zhao J, Nimaichand S, Xiao M, Li WJ.
タイトル Physiological and genomic properties of Thermus tenuipuniceus sp. nov., a novel slight reddish color member isolated from a terrestrial geothermal spring.
ジャーナル Syst Appl Microbiol
Abstract Two closely related, thermophilic bacteria, designated strains YIM 76954T and YIM 76947, were isolated from the Rehai Geothermal Field, Tengchong, Yunnan province, south-west China. Polyphasic approach and whole genome sequencing were used to determine the taxonomy status and genomic profiles of the novel strains. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the two isolates were closely related to Thermus scotoductus SE-1T (97.1% sequence similarity), and T. amyloliquefaciens YIM 77409T (96.6%). The strains could be differentiated from most recognized Thermus species by their whitish to slight reddish colony color, distinct DNA fingerprinting profiles and low ANI values. Cells stained Gram-negative, rod-shaped of diameter 0.2-0.5μm and length 1.5-5.0μm. Growth occurred at 50-75°C, pH 6.0-9.0 and in the presence of up to 1.0% (w/v) NaCl concentration. Thiosulfate was found to enhance cell growth, besides improving the intensity of its colony color. Oxygen, nitrate, sulfur, and Fe(III) could be used as terminal electron acceptors for growth. MK-8 was the major respiratory menaquinone. Major fatty acids were iso-C17:0, iso-C15:0, anteiso-C17:0, and anteiso-C15:0. The genome size was 2.26Mbp with 65.5% average GC content. A total of 2374 genes was predicted, comprising 2322 protein-coding and 52 RNA genes. On the basis of the polyphasic evidence presented, it is proposed that strain YIM 76954T represents a novel species of the genus Thermus, for which the name Thermus tenuipuniceus sp. nov. is proposed. The type strain is YIM 76954T (=JCM 30350T=KCTC 4677T).
巻・号 41(6)
ページ 611-618
公開日 2018-11-1
DOI 10.1016/j.syapm.2018.08.011
PII S0723-2020(18)30115-2
PMID 30217696
MeSH Bacterial Typing Techniques Base Composition China DNA Fingerprinting DNA, Bacterial / genetics Fatty Acids / chemistry Ferric Compounds / metabolism Geologic Sediments / microbiology Hot Springs / microbiology* Phospholipids / chemistry Phylogeny* Pigmentation RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Thermus / classification* Thermus / genetics Thermus / isolation & purification Vitamin K 2 / analogs & derivatives Vitamin K 2 / chemistry
IF 2.808
引用数 1
リソース情報
一般微生物 JCM 30350