RRC ID 55790
Author Etoh K, Fukuda M.
Title Rab10 regulates tubular endosome formation through KIF13A and KIF13B motors.
Journal J Cell Sci
Abstract Recycling endosomes are stations that sort endocytic cargoes to their appropriate destinations. Tubular endosomes have been characterized as a recycling endosomal compartment for clathrin-independent cargoes. However, the molecular mechanism by which tubular endosome formation is regulated is poorly understood. In this study, we identified Rab10 as a novel protein localized at tubular endosomes by using a comprehensive localization screen of EGFP-tagged Rab small GTPases. Knockout of Rab10 completely abolished tubular endosomal structures in HeLaM cells. We also identified kinesin motors KIF13A and KIF13B as novel Rab10-interacting proteins by means of in silico screening. The results of this study demonstrated that both the Rab10-binding homology domain and the motor domain of KIF13A are required for Rab10-positive tubular endosome formation. Our findings provide insight into the mechanism by which the Rab10-KIF13A (or KIF13B) complex regulates tubular endosome formation. This article has an associated First Person interview with the first author of the paper.
Volume 132(5)
Published 2019-2-19
DOI 10.1242/jcs.226977
PII jcs.226977
PMID 30700496
MeSH Endocytosis Endosomes / metabolism* Endosomes / ultrastructure Gene Knockout Techniques HeLa Cells Humans Kinesin / metabolism* Organelle Biogenesis* Protein Binding rab GTP-Binding Proteins / genetics rab GTP-Binding Proteins / metabolism*
IF 4.517
Times Cited 11
DNA material pmStr-C1-Rab10 (RDB17221) pMRX-puro-EGFP-Rab5A (RDB17222) pMRX-puro-EGFP-Rab10 (RDB17223) pMRX-puro-EGFP-Rab13 (RDB17224) pMRX-puro-EGFP-Rab17 (RDB17225) pMRX-puro-EGFP-Rab21 (RDB17226) pMRX-puro-EGFP-Rab22A (RDB17227) pMRX-puro-EGFP-Rab23 (RDB17228) pMRX-bsr-mStr-EMTB (RDB17229) pEGFP-C1-KIF13A-tail (RDB17230) pEGFP-C1-KIF13B-tail (RDB17231) pEGFP-C1-KIF13A-tail-deltaRHD (RDB17232) pEGFP-C1-KIF13B-tail-deltaRHD (RDB17233) pMRX-bsr-Myc-KIF13A (RDB17234) pMRX-puro-HA-KIF13B (RDB17235)