論文 - 詳細
| RRC ID | 5605 |
|---|---|
| 著者 | Reimer MM, Sörensen I, Kuscha V, Frank RE, Liu C, Becker CG, Becker T. |
| タイトル | Motor neuron regeneration in adult zebrafish. |
| ジャーナル | J Neurosci |
| Abstract |
The mammalian spinal cord does not regenerate motor neurons that are lost as a result of injury or disease. Here we demonstrate that adult zebrafish, which show functional spinal cord regeneration, are capable of motor neuron regeneration. After a spinal lesion, the ventricular zone shows a widespread increase in proliferation, including slowly proliferating olig2-positive (olig2+) ependymo-radial glial progenitor cells. Lineage tracing in olig2:green fluorescent protein transgenic fish indicates that these cells switch from a gliogenic phenotype to motor neuron production. Numbers of undifferentiated small HB9+ and islet-1+ motor neurons, which are double labeled with the proliferation marker 5-bromo-2-deoxyuridine (BrdU), are transiently strongly increased in the lesioned spinal cord. Large differentiated motor neurons, which are lost after a lesion, reappear at 6-8 weeks after lesion, and we detected ChAT+/BrdU+ motor neurons that were covered by contacts immunopositive for the synaptic marker SV2. These observations suggest that, after a lesion, plasticity of olig2+ progenitor cells may allow them to generate motor neurons, some of which exhibit markers for terminal differentiation and integration into the existing adult spinal circuitry. |
| 巻・号 | 28(34) |
| ページ | 8510-6 |
| 公開日 | 2008-8-20 |
| DOI | 10.1523/JNEUROSCI.1189-08.2008 |
| PII | 28/34/8510 |
| PMID | 18716209 |
| PMC | PMC6671064 |
| MeSH | Animals Animals, Genetically Modified Basic Helix-Loop-Helix Transcription Factors / genetics Basic Helix-Loop-Helix Transcription Factors / metabolism Bromodeoxyuridine Cell Count Cell Differentiation Cell Lineage Cell Proliferation Green Fluorescent Proteins / genetics Homeodomain Proteins / genetics Homeodomain Proteins / metabolism LIM-Homeodomain Proteins Microscopy, Electron Motor Neurons* / pathology Nerve Regeneration* Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism Neuroglia / metabolism Neuroglia / pathology Oligodendrocyte Transcription Factor 2 Phenotype Recombinant Fusion Proteins / genetics Spinal Cord / metabolism Spinal Cord / pathology Spinal Cord Injuries / pathology Spinal Cord Injuries / physiopathology* Stem Cells / metabolism Stem Cells / pathology Transcription Factors / genetics Transcription Factors / metabolism Zebrafish* Zebrafish Proteins / genetics Zebrafish Proteins / metabolism |
| IF | 5.674 |
| 引用数 | 151 |
| WOS 分野 | NEUROSCIENCES |
| リソース情報 | |
| ゼブラフィッシュ | Tg(isl1:GFP)rw0 |