RRC ID 5611
Author Ohata S, Kinoshita S, Aoki R, Tanaka H, Wada H, Tsuruoka-Kinoshita S, Tsuboi T, Watabe S, Okamoto H.
Title Neuroepithelial cells require fucosylated glycans to guide the migration of vagus motor neuron progenitors in the developing zebrafish hindbrain.
Journal Development
Abstract The molecular mechanisms by which neurons migrate and accumulate to form the neural layers and nuclei remain unclear. The formation of vagus motor nuclei in zebrafish embryos is an ideal model system in which to address this issue because of the transparency of the embryos and the availability of established genetic and molecular biological techniques. To determine the genes required for the formation of the vagus motor nuclei, we performed N-ethyl-N-nitrosourea-based mutant screening using a zebrafish line that expresses green fluorescent protein in the motor neurons. In wild-type embryos, the vagus motor neuron progenitors are born in the ventral ventricular zone, then migrate tangentially in the dorsolateral direction, forming the nuclei. However, in towhead (twd(rw685)) mutant embryos, the vagus motor neuron progenitors stray medially away from the normal migratory pathway and fail to stop in the right location. The twd(rw685) mutant has a defect in the GDP-mannose 4,6 dehydratase (gmds) gene, which encodes a key enzyme in the fucosylation pathway. Levels of fucosylated glycans were markedly and specifically reduced in twd(rw685) mutant embryos. Cell transplantation analysis revealed that GMDS is not essential in the vagus motor neuron progenitors for correct formation of the vagus motor nuclei, but is required in the neuroepithelial cells that surround the progenitors. Together, these findings suggest that fucosylated glycans expressed in neuroepithelial cells are required to guide the migration of vagus motor neuron progenitors.
Volume 136(10)
Pages 1653-63
Published 2009-5
DOI 10.1242/dev.033290
PII dev.033290
PMID 19369395
MeSH Amino Acid Sequence Animals Animals, Genetically Modified Body Patterning Cell Movement Embryo, Nonmammalian / cytology Embryo, Nonmammalian / physiology Green Fluorescent Proteins / biosynthesis Green Fluorescent Proteins / genetics Hydro-Lyases / genetics Molecular Sequence Data Motor Neurons / physiology* Mutation Neuroepithelial Cells / physiology* Polysaccharides / physiology* Rhombencephalon / cytology Rhombencephalon / embryology* Stem Cells / physiology* Vagus Nerve / cytology Vagus Nerve / embryology Vagus Nerve / physiology* Zebrafish / embryology Zebrafish / physiology*
IF 5.763
Times Cited 30
Zebrafish towhead rw685 Tg(CM-isl1:GFP)rw0