RRC ID 56137
著者 Ishibashi O, Akagi I, Ogawa Y, Inui T.
タイトル MiR-141-3p is upregulated in esophageal squamous cell carcinoma and targets pleckstrin homology domain leucine-rich repeat protein phosphatase-2, a negative regulator of the PI3K/AKT pathway.
ジャーナル Biochem Biophys Res Commun
Abstract The phosphatidylinositol-3-kinase (PI3K)/AKT pathway is frequently activated in various human cancers and plays essential roles in their development and progression. Accumulating evidence suggests that dysregulated expression of microRNAs (miRNAs) is closely associated with cancer progression and metastasis. Here, we focused on miRNAs that could regulate genes related to the PI3K/AKT pathway in esophageal squamous cell carcinoma (ESCC). To identify upregulated miRNAs and their possible target genes in ESCC, we performed microarray-based integrative analyses of miRNA and mRNA expression levels in three human ESCC cell lines and a normal esophageal epithelial cell line. The miRNA microarray analysis revealed that miR-31-5p, miR-141-3p, miR-200b-3p, miR-200c-3p, and miR-205-5p were expressed at higher levels in the ESCC cell lines than the normal esophageal epithelial cell line. Bioinformatical analyses of mRNA microarray data identified several AKT/PI3K pathway-related genes as candidate targets of these miRNAs, which include tumor suppressors such as DNA-damage-inducible transcript 4 and pleckstrin homology domain leucine-rich repeat protein phosphatase-2 (PHLPP2). To validate the targets of relevant miRNAs experimentally, synthetic mimics of the miRNAs were transfected into the esophageal epithelial cell line. Here, we report that miR-141-3p suppress the expression of PHLPP2, a negative regulators of the AKT/PI3K pathway, as a target in ESCC.
巻・号 501(2)
ページ 507-513
公開日 2018-6-22
DOI 10.1016/j.bbrc.2018.05.025
PII S0006-291X(18)31074-X
PMID 29738771
MeSH 3' Untranslated Regions Carcinoma, Squamous Cell / genetics* Carcinoma, Squamous Cell / metabolism Cell Line, Tumor Esophageal Neoplasms / genetics* Esophageal Neoplasms / metabolism Esophageal Squamous Cell Carcinoma Gene Expression Regulation, Neoplastic* Humans MicroRNAs / genetics* Phosphatidylinositol 3-Kinases / genetics* Phosphatidylinositol 3-Kinases / metabolism Phosphoprotein Phosphatases / genetics* Phosphoprotein Phosphatases / metabolism Proto-Oncogene Proteins c-akt / genetics* Proto-Oncogene Proteins c-akt / metabolism Signal Transduction Up-Regulation
IF 2.705
引用数 12
リソース情報
ヒト・動物細胞 TE-1(RCB1894) TE-5(RCB1949) TE-8(RCB2098)