RRC ID 56164
Author Akimoto T, Umemura M, Nagasako A, Ohtake M, Fujita T, Yokoyama U, Eguchi H, Yamamoto T, Ishikawa Y.
Title Alternating magnetic field enhances cytotoxicity of Compound C.
Journal Cancer Sci
Abstract We previously reported the efficacy of anti-cancer therapy with hyperthermia using an alternating magnetic field (AMF) and a magnetic compound. In the course of the study, unexpectedly, we found that an AMF enhances the cytotoxicity of Compound C, an activated protein kinase (AMPK) inhibitor, although this compound is not magnetic. Therefore, we examined the cellular mechanism of AMF-induced cytotoxicity of Compound C in cultured human glioblastoma (GB) cells. An AMF (280 kHz, 250 Arms) for 30 minutes significantly enhanced the cytotoxicity of Compound C and promoted apoptosis towards several human GB cell lines in vitro. The AMF also increased Compound C-induced cell-cycle arrest of GB cells at the G2 phase and, thus, inhibited cell proliferation. The AMF increased Compound C-induced reactive oxygen species production. Furthermore, the AMF decreased ERK phosphorylation in the presence of Compound C and suppressed the protective autophagy induced by this compound. The application of an AMF in cancer chemotherapy may be a simple and promising method, which might reduce the doses of drugs used in future cancer treatment and, therefore, the associated side effects.
Volume 109(11)
Pages 3483-3493
Published 2018-11-1
DOI 10.1111/cas.13781
PMID 30155931
PMC PMC6215876
MeSH Brain Neoplasms / drug therapy Brain Neoplasms / metabolism* Cell Line, Tumor Cell Proliferation / drug effects Cell Survival / drug effects Extracellular Signal-Regulated MAP Kinases / metabolism* Glioblastoma / drug therapy Glioblastoma / metabolism* Humans Hyperthermia, Induced Magnetic Fields Phosphorylation / drug effects Pyrazoles / pharmacology* Pyrimidines / pharmacology* Reactive Oxygen Species / metabolism
IF 4.751
Times Cited 4
Human and Animal Cells PANC-1(RCB2095)