RRC ID 5620
Author Mishima Y, Abreu-Goodger C, Staton AA, Stahlhut C, Shou C, Cheng C, Gerstein M, Enright AJ, Giraldez AJ.
Title Zebrafish miR-1 and miR-133 shape muscle gene expression and regulate sarcomeric actin organization.
Journal Genes Dev.
Abstract microRNAs (miRNAs) represent approximately 4% of the genes in vertebrates, where they regulate deadenylation, translation, and decay of the target messenger RNAs (mRNAs). The integrated role of miRNAs to regulate gene expression and cell function remains largely unknown. Therefore, to identify the targets coordinately regulated by muscle miRNAs in vivo, we performed gene expression arrays on muscle cells sorted from wild type, dicer mutants, and single miRNA knockdown embryos. Our analysis reveals that two particular miRNAs, miR-1 and miR-133, influence gene expression patterns in the zebrafish embryo where they account for >54% of the miRNA-mediated regulation in the muscle. We also found that muscle miRNA targets (1) tend to be expressed at low levels in wild-type muscle but are more highly expressed in dicer mutant muscle, and (2) are enriched for actin-related and actin-binding proteins. Loss of dicer function or down-regulation of miR-1 and miR-133 alters muscle gene expression and disrupts actin organization during sarcomere assembly. These results suggest that miR-1 and miR-133 actively shape gene expression patterns in muscle tissue, where they regulate sarcomeric actin organization.
Volume 23(5)
Pages 619-32
Published 2009-3-1
DOI 10.1101/gad.1760209
PII gad.1760209
PMID 19240126
PMC PMC2658521
MeSH Actins / metabolism* Animals Gene Expression Regulation, Developmental* Gene Knockdown Techniques MicroRNAs / metabolism* Muscle, Skeletal / metabolism* Mutation RNA, Messenger / metabolism Reproducibility of Results Sarcomeres / metabolism* Zebrafish / embryology* Zebrafish Proteins / genetics Zebrafish Proteins / metabolism
IF 8.99
Times Cited 87
WOS Category GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY CELL BIOLOGY
Resource
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