RRC ID 56201
Author Moriwaki T, Yamasaki A, Zhang-Akiyama QM.
Title ATM Induces Cell Death with Autophagy in Response to H2O2 Specifically in Caenorhabditis elegans Nondividing Cells.
Journal Oxid Med Cell Longev
Abstract Introduction:Ataxia-telangiectasia-mutated (ATM) kinase is a master regulator of the DNA damage response and is directly activated by reactive oxygen species (ROSs) in addition to DNA double-stranded breaks. However, the physiological function of the response to ROSs is not understood.
Purpose:In the present study, we investigated how ATM responds to ROSs in Caenorhabditis elegans (C. elegans).
Materials and Methods:First, we measured sensitivities of larvae to DNA-damaging agents and ROSs. Next, we analyzed the drug sensitivities of fully matured adult worms, which consist of nondividing somatic cells. Dead cell staining with acridine orange was performed to visualize the dead cells. In addition, we performed GFP reporter assays of lgg-1, an autophagy-related gene, to determine the types of cell death.
Results:atm-1(tm5027) larvae showed a wide range of sensitivities to both DNA-damaging agents and ROSs. In contrast, fully matured adult worms, which consist of nondividing somatic cells, showed sensitivity to DNA-damaging agent, NaHSO3, but they showed resistance to H2O2. Dead cell staining and GFP reporter assays of lgg-1 suggest that C. elegans ATM-1 induces the cell death with autophagy in intestinal cells in response to H2O2.
Conclusion:We revealed that ATM induces cell death in response to H2O2.
Volume 2018
Pages 3862070
Published 2018-7-2
DOI 10.1155/2018/3862070
PMID 30057676
PMC PMC6051064
MeSH Animals Ataxia Telangiectasia Mutated Proteins / genetics Ataxia Telangiectasia Mutated Proteins / metabolism* Autophagy / genetics Autophagy / physiology Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* DNA Damage / drug effects DNA Damage / genetics Hydrogen Peroxide / pharmacology Reactive Oxygen Species / metabolism Signal Transduction / genetics Signal Transduction / physiology
IF 4.868
Times Cited 2
C.elegans tm5027