Reference - Detail
|Author||Broitman-Maduro G, Owraghi M, Hung WW, Kuntz S, Sternberg PW, Maduro MF.|
|Title||The NK-2 class homeodomain factor CEH-51 and the T-box factor TBX-35 have overlapping function in C. elegans mesoderm development.|
The C. elegans MS blastomere, born at the 7-cell stage of embryogenesis, generates primarily mesodermal cell types, including pharynx cells, body muscles and coelomocytes. A presumptive null mutation in the T-box factor gene tbx-35, a target of the MED-1 and MED-2 divergent GATA factors, was previously found to result in a profound decrease in the production of MS-derived tissues, although the tbx-35(-) embryonic arrest phenotype was variable. We report here that the NK-2 class homeobox gene ceh-51 is a direct target of TBX-35 and at least one other factor, and that CEH-51 and TBX-35 share functions. Embryos homozygous for a ceh-51 null mutation arrest as larvae with pharynx and muscle defects, although these tissues appear to be specified correctly. Loss of tbx-35 and ceh-51 together results in a synergistic phenotype resembling loss of med-1 and med-2. Overexpression of ceh-51 causes embryonic arrest and generation of ectopic body muscle and coelomocytes. Our data show that TBX-35 and CEH-51 have overlapping function in MS lineage development. As T-box regulators and NK-2 homeodomain factors are both important for heart development in Drosophila and vertebrates, our results suggest that these regulators function in a similar manner in C. elegans to specify a major precursor of mesoderm.
|MeSH||Amino Acid Sequence Animals Animals, Genetically Modified Blastomeres / physiology Caenorhabditis elegans / anatomy & histology* Caenorhabditis elegans / embryology* Caenorhabditis elegans / growth & development Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Lineage Gene Expression Regulation, Developmental Gene Knockout Techniques Gene Regulatory Networks / physiology Homeodomain Proteins / genetics Homeodomain Proteins / metabolism* Mesoderm / physiology* Molecular Sequence Data Phenotype RNA Interference Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Sequence Alignment T-Box Domain Proteins / genetics T-Box Domain Proteins / metabolism* Transcription Factors / genetics Transcription Factors / metabolism*|
|WOS Category||DEVELOPMENTAL BIOLOGY|