RRC ID 57136
Author Aydogan MG, Wainman A, Saurya S, Steinacker TL, Caballe A, Novak ZA, Baumbach J, Muschalik N, Raff JW.
Title A homeostatic clock sets daughter centriole size in flies.
Journal J Cell Biol
Abstract Centrioles are highly structured organelles whose size is remarkably consistent within any given cell type. New centrioles are born when Polo-like kinase 4 (Plk4) recruits Ana2/STIL and Sas-6 to the side of an existing "mother" centriole. These two proteins then assemble into a cartwheel, which grows outwards to form the structural core of a new daughter. Here, we show that in early Drosophila melanogaster embryos, daughter centrioles grow at a linear rate during early S-phase and abruptly stop growing when they reach their correct size in mid- to late S-phase. Unexpectedly, the cartwheel grows from its proximal end, and Plk4 determines both the rate and period of centriole growth: the more active the centriolar Plk4, the faster centrioles grow, but the faster centriolar Plk4 is inactivated and growth ceases. Thus, Plk4 functions as a homeostatic clock, establishing an inverse relationship between growth rate and period to ensure that daughter centrioles grow to the correct size.
Volume 217(4)
Pages 1233-1248
Published 2018-4-2
DOI 10.1083/jcb.201801014
PII jcb.201801014
PMID 29500190
PMC PMC5881511
MeSH Animals Behavior, Animal Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Centrioles / enzymology* Centrioles / genetics Circadian Rhythm Signaling Peptides and Proteins / genetics Circadian Rhythm Signaling Peptides and Proteins / metabolism* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / embryology Drosophila melanogaster / enzymology* Drosophila melanogaster / genetics Embryo, Nonmammalian / enzymology Homeostasis Locomotion Microtubule-Associated Proteins / genetics Microtubule-Associated Proteins / metabolism Mutation Protein Binding Protein Transport Protein-Serine-Threonine Kinases / genetics Protein-Serine-Threonine Kinases / metabolism* S Phase* Signal Transduction Time Factors
IF 8.891
Times Cited 24
Resource
Drosophila