RRC ID 57531
Author Ito D, Zitouni S, Jana SC, Duarte P, Surkont J, Carvalho-Santos Z, Pereira-Leal JB, Ferreira MG, Bettencourt-Dias M.
Title Pericentrin-mediated SAS-6 recruitment promotes centriole assembly.
Journal Elife
Abstract The centrosome is composed of two centrioles surrounded by a microtubule-nucleating pericentriolar material (PCM). Although centrioles are known to regulate PCM assembly, it is less known whether and how the PCM contributes to centriole assembly. Here we investigate the interaction between centriole components and the PCM by taking advantage of fission yeast, which has a centriole-free, PCM-containing centrosome, the SPB. Surprisingly, we observed that several ectopically-expressed animal centriole components such as SAS-6 are recruited to the SPB. We revealed that a conserved PCM component, Pcp1/pericentrin, interacts with and recruits SAS-6. This interaction is conserved and important for centriole assembly, particularly its elongation. We further explored how yeasts kept this interaction even after centriole loss and showed that the conserved calmodulin-binding region of Pcp1/pericentrin is critical for SAS-6 interaction. Our work suggests that the PCM not only recruits and concentrates microtubule-nucleators, but also the centriole assembly machinery, promoting biogenesis close by.
Volume 8
Published 2019-6-11
DOI 10.7554/eLife.41418
PII 41418
PMID 31182187
PMC PMC6559791
MeSH Animals Animals, Genetically Modified Antigens / genetics Antigens / metabolism* Cells, Cultured Centrioles / metabolism* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / cytology Drosophila melanogaster / genetics Drosophila melanogaster / metabolism* Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Male Microscopy, Confocal Microtubules / metabolism Protein Binding Schizosaccharomyces / cytology Schizosaccharomyces / genetics Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Spermatozoa / cytology Spermatozoa / metabolism Time-Lapse Imaging / methods
IF 7.551
Times Cited 4
DNA material pDUAL2-HFG1c (RDB06157) pLYS1U-GFH21c (RDB06467) pLYS1U-HFY21c (RDB06475) pLYS1U-HFY1c (RDB06474)