Reference - Detail
|Author||Kawasaki Y, Miyamoto M, Oda T, Matsumura K, Negishi L, Nakato R, Suda S, Yokota N, Shirahige K, Akiyama T.|
|Title||The novel lncRNA CALIC upregulates AXL to promote colon cancer metastasis.|
Long non-coding RNAs (lncRNAs) are aberrantly expressed in many disease conditions, including cancer. Accumulating evidence indicates that some lncRNAs may play critical roles in cancer progression and metastasis. Here, we identify a set of lncRNAs that are upregulated in metastatic subpopulations isolated from colon cancer HCT116 cells in vivo and show that one of these lncRNAs, which we name CALIC, is required for the metastatic activity of colon cancer cells. We show that CALIC associates with the RNA-binding protein hnRNP-L and imparts specificity to hnRNP-L-mediated gene expression. Furthermore, we demonstrate that the CALIC/hnRNP-L complex upregulates the tyrosine kinase receptor AXL and that knockdown of CALIC or AXL using shRNA in colon cancer cells attenuates their ability to form metastases in mice. These results suggest that the CALIC/hnRNP-L complex enhances the metastatic potential of colon cancer cells.
|MeSH||Animals Cell Proliferation Colonic Neoplasms / genetics* Colonic Neoplasms / metabolism Colonic Neoplasms / mortality Colonic Neoplasms / pathology Disease Progression Female Gene Expression Regulation, Neoplastic* HCT116 Cells Humans Liver Neoplasms / genetics* Liver Neoplasms / metabolism Liver Neoplasms / mortality Liver Neoplasms / secondary Lymphatic Metastasis Mice Mice, Inbred BALB C Mice, Nude Protein Binding Proto-Oncogene Proteins / antagonists & inhibitors Proto-Oncogene Proteins / genetics* Proto-Oncogene Proteins / metabolism RNA, Long Noncoding / antagonists & inhibitors RNA, Long Noncoding / genetics* RNA, Long Noncoding / metabolism RNA, Small Interfering / genetics RNA, Small Interfering / metabolism Receptor Protein-Tyrosine Kinases / antagonists & inhibitors Receptor Protein-Tyrosine Kinases / genetics* Receptor Protein-Tyrosine Kinases / metabolism Ribonucleoproteins / genetics* Ribonucleoproteins / metabolism Signal Transduction Survival Analysis Xenograft Model Antitumor Assays|
|DNA material||CS-RfA-CG (RDB04390) pCMV-VSV-G-RSV-Rev (RDB04393) pCAG-HIVgp (RDB04394)|
|Human and Animal Cells||HCT116(RCB2979)|