RRC ID 57608
Author Wang B, Lin J, Zhang Q, Zhang X, Yu H, Gong P, Xiang L.
Title αCGRP Affects BMSCs' Migration and Osteogenesis via the Hippo-YAP Pathway.
Journal Cell Transplant
Abstract Alpha-calcitonin gene-related peptide (αCGRP) plays a significant pathophysiological role in the regulation of bone metabolism. Our previous research indicated that αCGRP might have a potential application in enhancing osseointegration in vivo. To further uncover the intrinsic mechanism of its networks in bone regeneration, here we investigate the impact of αCGRP on osteogenic differentiation in bone marrow-derived mesenchymal stem cells (BMSCs) from both wild-type and αCGRP-/- mice. Considering the half-life of αCGRP in plasma is only 10 min, we applied αCGRP lentivirus and stably transfected it into BMSCs, followed by transfection identification and cell cycle assay. We further conducted a series of in vitro tests, and the results revealed that biological functions including migratory ability and osteogenicity exhibited positive correlation with BMSCs' αCGRP expression. Meanwhile, this phenomenon was associated with an enhanced expression of YAP (Yes-associated protein), the key downstream effector of the Hippo pathway. To sum up, our data together with previous in vivo observations is likely to elucidate the intrinsic mechanism of αCGRP in bone remodeling, and αCGRP would appear to be a novel treatment to promote bone wound healing.
Volume 28(11)
Pages 1420-1431
Published 2019-11-1
DOI 10.1177/0963689719871000
PMID 31426665
PMC PMC6802143
MeSH Adaptor Proteins, Signal Transducing / genetics Adaptor Proteins, Signal Transducing / metabolism* Animals Bone Regeneration / drug effects Bone Regeneration / genetics Calcitonin Gene-Related Peptide / genetics* Calcitonin Gene-Related Peptide / metabolism Cell Cycle Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Cell Movement / drug effects Cell Movement / genetics* Cells, Cultured Gene Expression Genetic Vectors Lentivirus / genetics Mesenchymal Stem Cells / cytology Mesenchymal Stem Cells / metabolism* Mice Mice, Knockout Osteogenesis / drug effects Osteogenesis / genetics* YAP-Signaling Proteins
IF 3.477
Times Cited 0
Mice RBRC04109