RRC ID 57698
Author Chang J, Xu Z, Li M, Yang M, Qin H, Yang J, Wu S.
Title Spatiotemporal cytoskeleton organizations determine morphogenesis of multicellular trichomes in tomato.
Journal PLoS Genet.
Abstract Plant trichomes originate from epidermal cell, forming protective structure from abiotic and biotic stresses. Different from the unicellular trichome in Arabidopsis, tomato trichomes are multicellular structure and can be classified into seven different types based on cell number, shape and the presence of glandular cells. Despite the importance of tomato trichomes in insect resistance, our understanding of the tomato trichome morphogenesis remains elusive. In this study, we quantitatively analyzed morphological traits of trichomes in tomato and further performed live imaging of cytoskeletons in stably transformed lines with actin and microtubule markers. At different developmental stages, two types of cytoskeletons exhibited distinct patterns in different trichome cells, ranging from transverse, spiral to longitudinal. This gradual transition of actin filament angle from basal to top cells could correlate with the spatial expansion mode in different cells. Further genetic screen for aberrant trichome morphology led to the discovery of a number of independent mutations in SCAR/WAVE and ARP2/3 complex, which resulted in actin bundling and distorted trichomes. Disruption of microtubules caused isotropic expansion while abolished actin filaments entirely inhibited axial extension of trichomes, indicating that microtubules and actin filaments may control distinct aspects of trichome cell expansion. Our results shed light on the roles of cytoskeletons in the formation of multicellular structure of tomato trichomes.
Volume 15(10)
Pages e1008438
Published 2019-10
DOI 10.1371/journal.pgen.1008438
PII PGENETICS-D-19-00437
PMID 31584936
IF 5.224
Resource
Tomato TOMJPF00001(Micro-Tom) TOMJPE7376-1 TOMJPE3936-2 TOMJPW3695-1 TOMJPW4375 TOMJPW2352-1 TOMJPW2193-1