RRC ID 57705
Author Abbasi F, Miyata H, Shimada K, Morohoshi A, Nozawa K, Matsumura T, Xu Z, Pratiwi P, Ikawa M.
Title RSPH6A is required for sperm flagellum formation and male fertility in mice.
Journal J Cell Sci
Abstract The flagellum is an evolutionarily conserved appendage used for sensing and locomotion. Its backbone is the axoneme and a component of the axoneme is the radial spoke (RS), a protein complex implicated in flagellar motility regulation. Numerous diseases occur if the axoneme is improperly formed, such as primary ciliary dyskinesia (PCD) and infertility. Radial spoke head 6 homolog A (RSPH6A) is an ortholog of Chlamydomonas RSP6 in the RS head and is evolutionarily conserved. While some RS head proteins have been linked to PCD, little is known about RSPH6A. Here, we show that mouse RSPH6A is testis-enriched and localized in the flagellum. Rsph6a knockout (KO) male mice are infertile as a result of their short immotile spermatozoa. Observation of the KO testis indicates that the axoneme can elongate but is disrupted before accessory structures are formed. Manchette removal is also impaired in the KO testis. Further, RSPH9, another radial spoke protein, disappeared in the Rsph6a KO flagella. These data indicate that RSPH6A is essential for sperm flagellar assembly and male fertility in mice.This article has an associated First Person interview with the first author of the paper.
Volume 131(19)
Published 2018-10-11
DOI 10.1242/jcs.221648
PII jcs.221648
PMID 30185526
PMC PMC6198453
MeSH Animals Base Sequence CRISPR-Cas Systems / genetics Conserved Sequence Evolution, Molecular Fertility* Flagella / metabolism* Flagella / ultrastructure HEK293 Cells Humans Male Mice Mice, Mutant Strains Mitochondria / metabolism Organ Specificity Phenotype Protein Binding Protein Transport Proteins / metabolism* Sperm Injections, Intracytoplasmic Sperm Tail / metabolism Spermatozoa / metabolism* Spermatozoa / ultrastructure Testis / metabolism Tubulin / metabolism
IF 4.517
Times Cited 11
DNA material pCAG_mRsph1-PA (RDB17449) pCAG_mRsph4a-PA (RDB17450) pCAG_mRsph9-PA (RDB17451) pCAG_mRsph10b-PA (RDB17452) pCAG_mRsph6a-FLAG (RDB17453)