| RRC ID |
57705
|
| 著者 |
Abbasi F, Miyata H, Shimada K, Morohoshi A, Nozawa K, Matsumura T, Xu Z, Pratiwi P, Ikawa M.
|
| タイトル |
RSPH6A is required for sperm flagellum formation and male fertility in mice.
|
| ジャーナル |
J Cell Sci
|
| Abstract |
The flagellum is an evolutionarily conserved appendage used for sensing and locomotion. Its backbone is the axoneme and a component of the axoneme is the radial spoke (RS), a protein complex implicated in flagellar motility regulation. Numerous diseases occur if the axoneme is improperly formed, such as primary ciliary dyskinesia (PCD) and infertility. Radial spoke head 6 homolog A (RSPH6A) is an ortholog of Chlamydomonas RSP6 in the RS head and is evolutionarily conserved. While some RS head proteins have been linked to PCD, little is known about RSPH6A. Here, we show that mouse RSPH6A is testis-enriched and localized in the flagellum. Rsph6a knockout (KO) male mice are infertile as a result of their short immotile spermatozoa. Observation of the KO testis indicates that the axoneme can elongate but is disrupted before accessory structures are formed. Manchette removal is also impaired in the KO testis. Further, RSPH9, another radial spoke protein, disappeared in the Rsph6a KO flagella. These data indicate that RSPH6A is essential for sperm flagellar assembly and male fertility in mice.This article has an associated First Person interview with the first author of the paper.
|
| 巻・号 |
131(19)
|
| 公開日 |
2018-10-11
|
| DOI |
10.1242/jcs.221648
|
| PII |
jcs.221648
|
| PMID |
30185526
|
| PMC |
PMC6198453
|
| MeSH |
Animals
Base Sequence
CRISPR-Cas Systems / genetics
Conserved Sequence
Evolution, Molecular
Fertility*
Flagella / metabolism*
Flagella / ultrastructure
HEK293 Cells
Humans
Male
Mice
Mice, Mutant Strains
Mitochondria / metabolism
Organ Specificity
Phenotype
Protein Binding
Protein Transport
Proteins / metabolism*
Sperm Injections, Intracytoplasmic
Sperm Tail / metabolism
Spermatozoa / metabolism*
Spermatozoa / ultrastructure
Testis / metabolism
Tubulin / metabolism
|
| IF |
4.573
|
| 引用数 |
11
|
| リソース情報 |
| 遺伝子材料 |
pCAG_mRsph1-PA (RDB17449)
pCAG_mRsph4a-PA (RDB17450)
pCAG_mRsph9-PA (RDB17451)
pCAG_mRsph10b-PA (RDB17452)
pCAG_mRsph6a-FLAG (RDB17453) |
