Reference - Detail
| RRC ID | 57747 |
|---|---|
| Author | Chu P, Wang Q, Wang Z, Gao C. |
| Title | Long non-coding RNA highly up-regulated in liver cancer protects tumor necrosis factor-alpha-induced inflammatory injury by down-regulation of microRNA-101 in ATDC5 cells. |
| Journal | Int Immunopharmacol |
| Abstract |
BACKGROUND:Osteoarthritis (OA) is a familiar joint degenerative disease. Long non-coding RNAs (lncRNAs) play vital roles in the pathogenesis of OA. Nevertheless, the regulatory impacts of lncRNA highly up-regulated in liver cancer (lncRNA-HULC) on OA remain dimness. The study tried to probe the protective effect of HULC on ATDC5 cells against tumor necrosis factor-alpha (TNF-α)-induced inflammatory injury. METHODS:Relative expression levels of pro-inflammatory cytokines (IL-6, IL-8 and MCP-1) and HULC in OA cartilage tissues and normal cartilage tissues were determined by RT-qPCR. TNF-α induced inflammatory injury model in ATDC5 cells was constructed, and the biological functions of HULC overexpression or suppression in TNF-α-injured ATDC5 cells were assessed. The relevancy between miR-101 and HULC was investigated by utilizing bioinformatics prediction, luciferase reporter assay, RNA pull-down and immunoprecipitation. MiR-101 mimic and inhibitor were transfected into ATDC5 cells, and its regulatory effect on TNF-α-injured ATDC5 cells was examined. Further, NF-κB and MAPK signaling pathways were finally detected by western blot. RESULTS:Enhancement of IL-6, IL-8 and MCP-1 were observated in OA cartilage tissues, but repression of HULC was discovered in OA cartilage tissues. HULC expression was decreased by TNF-α treatment, and overexpressed HULC significantly relieved TNF-α-induced ATDC5 cells injury. Additionally, miR-101 was mutual repressed with HULC, and overexpressed miR-101 reversed the protective effect of HULC in TNF-α-injured ATDC5 cells. Besides, HULC blocked NF-κB and MAPK pathways via repression of miR-101. CONCLUSIONS:The discoveries testified that HULC protected ATDC5 cells against TNF-α-induced inflammatory injury by repression of miR-101. |
| Volume | 72 |
| Pages | 148-158 |
| Published | 2019-7-1 |
| DOI | 10.1016/j.intimp.2019.04.004 |
| PII | S1567-5769(18)30689-1 |
| PMID | 30981080 |
| MeSH | Animals Cartilage, Articular / cytology Cartilage, Articular / immunology Cells, Cultured Chondrocytes / immunology* Cytokines / immunology* Down-Regulation Female Humans Male Mice MicroRNAs / genetics MicroRNAs / immunology* Middle Aged Osteoarthritis / genetics Osteoarthritis / immunology* RNA, Long Noncoding / genetics RNA, Long Noncoding / immunology* |
| IF | 3.943 |
| Times Cited | 4 |
| Resource | |
| Human and Animal Cells | ATDC5(RCB0565) |