論文 - 詳細
| RRC ID | 5775 |
|---|---|
| 著者 | Yoshinari S, Shiba T, Inaoka DK, Itoh T, Kurisu G, Harada S, Kita K, Watanabe Y. |
| タイトル | Functional importance of crenarchaea-specific extra-loop revealed by an X-ray structure of a heterotetrameric crenarchaeal splicing endonuclease. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
Archaeal splicing endonucleases (EndAs) are currently classified into three groups. Two groups require a single subunit protein to form a homodimer or homotetramer. The third group requires two nonidentical protein components for the activity. To elucidate the molecular architecture of the two-subunit EndA system, we studied a crenarchaeal splicing endonuclease from Pyrobaculum aerophilum. In the present study, we solved a crystal structure of the enzyme at 1.7-A resolution. The enzyme adopts a heterotetrameric form composed of two catalytic and two structural subunits. By connecting the structural and the catalytic subunits of the heterotetrameric EndA, we could convert the enzyme to a homodimer that maintains the broad substrate specificity that is one of the characteristics of heterotetrameric EndA. Meanwhile, a deletion of six amino acids in a Crenarchaea-specific loop abolished the endonuclease activity even on a substrate with canonical BHB motif. These results indicate that the subunit architecture is not a major factor responsible for the difference of substrate specificity between single- and two-subunit EndA systems. Rather, the structural basis for the broad substrate specificity is built into the crenarchaeal splicing endonuclease itself. |
| 巻・号 | 37(14) |
| ページ | 4787-98 |
| 公開日 | 2009-8-1 |
| DOI | 10.1093/nar/gkp506 |
| PII | gkp506 |
| PMID | 19515941 |
| PMC | PMC2724299 |
| MeSH | Amino Acid Sequence Crystallography, X-Ray Endoribonucleases / chemistry* Endoribonucleases / genetics Endoribonucleases / metabolism Models, Molecular Molecular Sequence Data Protein Engineering Protein Multimerization Protein Subunits / chemistry Pyrobaculum / enzymology* Sequence Deletion Substrate Specificity |
| IF | 11.502 |
| 引用数 | 17 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 一般微生物 | JCM9630 |