| RRC ID |
57832
|
| 著者 |
Li X, Yu M, Han L, Chen L, Zhang D, Zhou G, Zhao Q, Sun T.
|
| タイトル |
LINC00305 represses miR-124 expression to trigger inflammatory insults in the presence of lipopolysaccharide.
|
| ジャーナル |
Artif Cells Nanomed Biotechnol
|
| Abstract |
Background: The anti-inflammatory function of microRNA-124 (miR-124) has been a matter of extensive studies in the last few years. Although LINC00305 regulates biological activities by acting as a miR sponge, it is still unexplored whether LINC00305 is involved in inflammation by regulating miR-124. Methods: Cell viability and apoptosis were evaluated with commercial kits, cell counting kit-8 (CCK-8) and Annexin V-fluorescein isothiocyanate (FITC) kit, respectively. LINC00305, miR-124 and mRNA levels were quantified by quantitative reverse transcription PCR (qRT-PCR). Protein level was assessed with Western blot assay and enzyme-linked immunosorbent assay (ELISA). Results: The expression of LINC00305 was up-regulated by lipopolysaccharide (LPS). LINC00305 overexpression further suppressed the cell viability, promoted apoptosis and induced inflammation in LPS-treated ATDC5 cells while its silence enhanced the cell viability, inhibited apoptosis and ameliorated inflammation. miR-124 was negatively regulated by LINC00305 and its overexpression abolished the effects of LINC00305 in the presence of LPS. LINC00305 further triggered the Notch/nuclear factor kappa B (NF-κB) signalling pathway in LPS-treated ATDC5 cells and this activation was abrogated when ATDC5 cells overexpressed miR-124. Conclusion: LINC00305 might emerge as a novel suppressor for miR-124. LINC00305-caused miR-124 silence compromises ATDC5 cell viability and ultimately results in inflammatory insults by activating Notch/NF-κB pathway.
|
| 巻・号 |
47(1)
|
| ページ |
2352-2360
|
| 公開日 |
2019-12-1
|
| DOI |
10.1080/21691401.2019.1624559
|
| PMID |
31190561
|
| MeSH |
Cell Line
Down-Regulation / drug effects
Down-Regulation / genetics
Gene Expression Regulation / drug effects*
Gene Expression Regulation / genetics*
Humans
Inflammation / genetics
Lipopolysaccharides / pharmacology*
MicroRNAs / genetics*
NF-kappa B / metabolism
RNA, Long Noncoding / genetics*
Receptors, Notch / metabolism
Signal Transduction / drug effects
Signal Transduction / genetics
|
| IF |
3.343
|
| 引用数 |
2
|
| リソース情報 |
| ヒト・動物細胞 |
ATDC5(RCB0565) |
