論文 - 詳細
| RRC ID | 5802 |
|---|---|
| 著者 | Tang L, Machacek T, Mamnun YM, Penkner A, Gloggnitzer J, Wegrostek C, Konrat R, Jantsch MF, Loidl J, Jantsch V. |
| タイトル | Mutations in Caenorhabditis elegans him-19 show meiotic defects that worsen with age. |
| ジャーナル | Mol Biol Cell |
| Abstract |
From a screen for meiotic Caenorhabditis elegans mutants based on high incidence of males, we identified a novel gene, him-19, with multiple functions in prophase of meiosis I. Mutant him-19(jf6) animals show a reduction in pairing of homologous chromosomes and subsequent bivalent formation. Consistently, synaptonemal complex formation is spatially restricted and possibly involves nonhomologous chromosomes. Also, foci of the recombination protein RAD-51 occur delayed or cease altogether. Ultimately, mutation of him-19 leads to chromosome missegregation and reduced offspring viability. The observed defects suggest that HIM-19 is important for both homology recognition and formation of meiotic DNA double-strand breaks. It therefore seems to be engaged in an early meiotic event, resembling in this respect the regulator kinase CHK-2. Most astonishingly, him-19(jf6) hermaphrodites display worsening of phenotypes with increasing age, whereas defects are more severe in female than in male meiosis. This finding is consistent with depletion of a him-19-dependent factor during the production of oocytes. Further characterization of him-19 could contribute to our understanding of age-dependent meiotic defects in humans. |
| 巻・号 | 21(6) |
| ページ | 885-96 |
| 公開日 | 2010-3-15 |
| DOI | 10.1091/mbc.e09-09-0811 |
| PII | E09-09-0811 |
| PMID | 20071466 |
| PMC | PMC2836969 |
| MeSH | Amino Acid Sequence Animals Caenorhabditis elegans / anatomy & histology Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism* Chromosome Pairing / genetics DNA Breaks, Double-Stranded DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Female Gonads / anatomy & histology Humans Male Meiosis / physiology* Molecular Sequence Data Mutation* Oogenesis / physiology Phenotype RNA Splicing Recombination, Genetic |
| IF | 3.791 |
| 引用数 | 14 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 線虫 | tm3538 |