Reference - Detail
| RRC ID | 58321 |
|---|---|
| Author | Hatsuzawa K, Hirose H, Tani K, Yamamoto A, Scheller RH, Tagaya M. |
| Title | Syntaxin 18, a SNAP receptor that functions in the endoplasmic reticulum, intermediate compartment, and cis-Golgi vesicle trafficking. |
| Journal | J Biol Chem |
| Abstract |
Members of the syntaxin family are target-soluble N-ethylmaleimide-sensitive factor-attachment protein receptors involved in vesicle docking and/or fusion within the exocytic and endocytotic pathways. By using the yeast two-hybrid system, we have identified a novel member of the syntaxin family, syntaxin 18, that binds to alpha-soluble N-ethylmaleimide-sensitive factor-attachment protein. Subcellular fractionation and immunocytochemical analysis revealed that syntaxin 18 is principally located in the endoplasmic reticulum. We examined the effect of overexpression of FLAG-tagged syntaxin 18 and a mutant lacking the N-terminal 81 amino acid residues on protein transport and organelles in the early secretory pathway. Both expressed proteins localized to the endoplasmic reticulum, and the expressed FLAG-syntaxin 18 caused remarkable aggregation of endoplasmic reticulum membranes. Although expression of the FLAG-syntaxin 18 lacking the N-terminal region produced less effect on the morphology of the endoplasmic reticulum, dispersion of the endoplasmic reticulum-Golgi intermediate compartment and cis-Golgi was elicited. Moreover, overexpression of the FLAG-syntaxin 18 mutant inhibited protein export from the endoplasmic reticulum. These results taken together suggest that syntaxin 18 functions in transport between the endoplasmic reticulum and Golgi. |
| Volume | 275(18) |
| Pages | 13713-20 |
| Published | 2000-5-5 |
| DOI | 10.1074/jbc.275.18.13713 |
| PII | S0021-9258(19)80742-1 |
| PMID | 10788491 |
| MeSH | Amino Acid Sequence Animals Base Sequence Biological Transport Cytoplasmic Granules / metabolism Endoplasmic Reticulum / metabolism* Golgi Apparatus / metabolism* HeLa Cells Humans Liver / metabolism Liver / ultrastructure Membrane Proteins / genetics Membrane Proteins / metabolism* Molecular Sequence Data Nerve Tissue Proteins / metabolism Qa-SNARE Proteins Rats Rats, Wistar SNARE Proteins Sequence Alignment Vesicular Transport Proteins* |
| IF | 4.238 |
| Times Cited | 83 |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | syntaxin18 (RDB03042) |
| Human and Animal Cells | HeLa(RCB0007) |