RRC ID 58321
著者 Hatsuzawa K, Hirose H, Tani K, Yamamoto A, Scheller RH, Tagaya M.
タイトル Syntaxin 18, a SNAP receptor that functions in the endoplasmic reticulum, intermediate compartment, and cis-Golgi vesicle trafficking.
ジャーナル J Biol Chem
Abstract Members of the syntaxin family are target-soluble N-ethylmaleimide-sensitive factor-attachment protein receptors involved in vesicle docking and/or fusion within the exocytic and endocytotic pathways. By using the yeast two-hybrid system, we have identified a novel member of the syntaxin family, syntaxin 18, that binds to alpha-soluble N-ethylmaleimide-sensitive factor-attachment protein. Subcellular fractionation and immunocytochemical analysis revealed that syntaxin 18 is principally located in the endoplasmic reticulum. We examined the effect of overexpression of FLAG-tagged syntaxin 18 and a mutant lacking the N-terminal 81 amino acid residues on protein transport and organelles in the early secretory pathway. Both expressed proteins localized to the endoplasmic reticulum, and the expressed FLAG-syntaxin 18 caused remarkable aggregation of endoplasmic reticulum membranes. Although expression of the FLAG-syntaxin 18 lacking the N-terminal region produced less effect on the morphology of the endoplasmic reticulum, dispersion of the endoplasmic reticulum-Golgi intermediate compartment and cis-Golgi was elicited. Moreover, overexpression of the FLAG-syntaxin 18 mutant inhibited protein export from the endoplasmic reticulum. These results taken together suggest that syntaxin 18 functions in transport between the endoplasmic reticulum and Golgi.
巻・号 275(18)
ページ 13713-20
公開日 2000-5-5
DOI 10.1074/jbc.275.18.13713
PII S0021-9258(19)80742-1
PMID 10788491
MeSH Amino Acid Sequence Animals Base Sequence Biological Transport Cytoplasmic Granules / metabolism Endoplasmic Reticulum / metabolism* Golgi Apparatus / metabolism* HeLa Cells Humans Liver / metabolism Liver / ultrastructure Membrane Proteins / genetics Membrane Proteins / metabolism* Molecular Sequence Data Nerve Tissue Proteins / metabolism Qa-SNARE Proteins Rats Rats, Wistar SNARE Proteins Sequence Alignment Vesicular Transport Proteins*
IF 4.238
引用数 83
リソース情報
遺伝子材料 syntaxin18 (RDB03042)
ヒト・動物細胞 HeLa(RCB0007)