RRC ID |
58321
|
著者 |
Hatsuzawa K, Hirose H, Tani K, Yamamoto A, Scheller RH, Tagaya M.
|
タイトル |
Syntaxin 18, a SNAP receptor that functions in the endoplasmic reticulum, intermediate compartment, and cis-Golgi vesicle trafficking.
|
ジャーナル |
J Biol Chem
|
Abstract |
Members of the syntaxin family are target-soluble N-ethylmaleimide-sensitive factor-attachment protein receptors involved in vesicle docking and/or fusion within the exocytic and endocytotic pathways. By using the yeast two-hybrid system, we have identified a novel member of the syntaxin family, syntaxin 18, that binds to alpha-soluble N-ethylmaleimide-sensitive factor-attachment protein. Subcellular fractionation and immunocytochemical analysis revealed that syntaxin 18 is principally located in the endoplasmic reticulum. We examined the effect of overexpression of FLAG-tagged syntaxin 18 and a mutant lacking the N-terminal 81 amino acid residues on protein transport and organelles in the early secretory pathway. Both expressed proteins localized to the endoplasmic reticulum, and the expressed FLAG-syntaxin 18 caused remarkable aggregation of endoplasmic reticulum membranes. Although expression of the FLAG-syntaxin 18 lacking the N-terminal region produced less effect on the morphology of the endoplasmic reticulum, dispersion of the endoplasmic reticulum-Golgi intermediate compartment and cis-Golgi was elicited. Moreover, overexpression of the FLAG-syntaxin 18 mutant inhibited protein export from the endoplasmic reticulum. These results taken together suggest that syntaxin 18 functions in transport between the endoplasmic reticulum and Golgi.
|
巻・号 |
275(18)
|
ページ |
13713-20
|
公開日 |
2000-5-5
|
DOI |
10.1074/jbc.275.18.13713
|
PII |
S0021-9258(19)80742-1
|
PMID |
10788491
|
MeSH |
Amino Acid Sequence
Animals
Base Sequence
Biological Transport
Cytoplasmic Granules / metabolism
Endoplasmic Reticulum / metabolism*
Golgi Apparatus / metabolism*
HeLa Cells
Humans
Liver / metabolism
Liver / ultrastructure
Membrane Proteins / genetics
Membrane Proteins / metabolism*
Molecular Sequence Data
Nerve Tissue Proteins / metabolism
Qa-SNARE Proteins
Rats
Rats, Wistar
SNARE Proteins
Sequence Alignment
Vesicular Transport Proteins*
|
IF |
4.238
|
引用数 |
83
|
リソース情報 |
遺伝子材料 |
syntaxin18 (RDB03042) |
ヒト・動物細胞 |
HeLa(RCB0007) |