Reference - Detail
| RRC ID | 58437 |
|---|---|
| Author | Wang P, Liu H, Wang Y, Liu O, Zhang J, Gleason A, Yang Z, Wang H, Shi A, Grant BD. |
| Title | RAB-10 Promotes EHBP-1 Bridging of Filamentous Actin and Tubular Recycling Endosomes. |
| Journal | PLoS Genet |
| Abstract |
EHBP-1 (Ehbp1) is a conserved regulator of endocytic recycling, acting as an effector of small GTPases including RAB-10 (Rab10). Here we present evidence that EHBP-1 associates with tubular endosomal phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2] enriched membranes through an N-terminal C2-like (NT-C2) domain, and define residues within the NT-C2 domain that mediate membrane interaction. Furthermore, our results indicate that the EHBP-1 central calponin homology (CH) domain binds to actin microfilaments in a reaction that is stimulated by RAB-10(GTP). Loss of any aspect of this RAB-10/EHBP-1 system in the C. elegans intestinal epithelium leads to retention of basolateral recycling cargo in endosomes that have lost their normal tubular endosomal network (TEN) organization. We propose a mechanism whereby RAB-10 promotes the ability of endosome-bound EHBP-1 to also bind to the actin cytoskeleton, thereby promoting endosomal tubulation. |
| Volume | 12(6) |
| Pages | e1006093 |
| Published | 2016-6-1 |
| DOI | 10.1371/journal.pgen.1006093 |
| PII | PGENETICS-D-15-03079 |
| PMID | 27272733 |
| PMC | PMC4894640 |
| MeSH | Actin Cytoskeleton / metabolism Actins / metabolism* Animals Biological Transport / physiology Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / metabolism* Endocytosis / physiology Endosomes / metabolism* Intestinal Mucosa / metabolism Phosphatidylinositol 4,5-Diphosphate / metabolism Protein Binding / physiology Protein Transport / physiology Vesicular Transport Proteins / metabolism* rab GTP-Binding Proteins / metabolism* |
| IF | 5.175 |
| Times Cited | 18 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm2523 tm1447 |