RRC ID 5857
Author Inohaya K, Takano Y, Kudo A.
Title Production of Wnt4b by floor plate cells is essential for the segmental patterning of the vertebral column in medaka.
Journal Development
Abstract The floor plate is a key organizer that controls the specification of neurons in the central nervous system. Here, we show a new role of the floor plate: segmental pattern formation of the vertebral column. Analysis of a spontaneous medaka mutant, fused centrum (fsc), which exhibits fused centra and the absence of the intervertebral ligaments, revealed that fsc encodes wnt4b, which was expressed exclusively in the floor plate. In fsc mutants, we found that wnt4b expression was completely lost in the floor plate and that abnormal conversion of the intervertebral ligament cells into osteoblasts appeared to cause a defect of the intervertebral ligaments. The establishment of the transgenic rescue lines and mosaic analyses allowed the conclusion to be drawn that production of wnt4b by floor plate cells is essential for the segmental patterning of the vertebral column. Our findings provide a novel perspective on the mechanism of vertebrate development.
Volume 137(11)
Pages 1807-13
Published 2010-6
DOI 10.1242/dev.051540
PII 137/11/1807
PMID 20460365
IF 5.413
Times Cited 23
WOS Category DEVELOPMENTAL BIOLOGY
Resource
Medaka medaka Hd-rR fosmid DNA Qurt (MT828) HNI-II (IB176) fu (MT51) Fused(BBRRfufu) (MT1122) wnt4b-EGFP (TG1259) wnt4b^fsc/fsc-Tg(wnt4b-zfcry:EGFP) (TG1260)