RRC ID 58656
Author Tachibana K, Ohnishi H, Ali Haghparast SM, Kihara T, Miyake J.
Title Activation of PKC induces leukocyte adhesion by the dephosphorylation of ERM.
Journal Biochem Biophys Res Commun
Abstract Although circulating leukocytes are non-adherent cells, they also undergo adhesion in response to external stimuli. To elucidate this switch mechanism, we investigated PMA-induced cell adhesion in myelomonocytic KG-1 cells. PMA induced microvillius collapse, decrease of cell surface rigidity and exclusion of sialomucin from adhesion sites. All these adhesion-contributing events are linked to dephosphorylation of Ezrin/Radixin/Moesin (ERM) proteins. Indeed, PMA-treatment induced quick decrease of phosphorylated ERM proteins, while expression of Moesin-T558D, a phospho-mimetic mutant, inhibited PMA-induced cell adhesion. PMA-induced cell adhesion and ERM-dephophorylation were inhibited by PKC inhibitors or by a phosphatase inhibitor, indicating the involvement of PKC and protein phophatase in these processes. In peripheral T lymphocytes, ERM-dephosphorylation by adhesion-inducing stimuli was inhibited by a PKC inhibitor. Combined, these findings strongly suggest that external stimuli induce ERM-dephosphorylation via the activation of PKC in leukocytes and that ERM-dephosphorylation leads to leukocytes' adhesion.
Volume 523(1)
Pages 177-182
Published 2020-2-26
DOI 10.1016/j.bbrc.2019.12.044
PII S0006-291X(19)32364-2
PMID 31843195
MeSH Cell Adhesion / drug effects* Cell Line Cytoskeletal Proteins / chemistry Cytoskeletal Proteins / metabolism* Dose-Response Relationship, Drug Enzyme Activation / drug effects Humans Leukocytes / cytology* Leukocytes / drug effects* Leukocytes / metabolism Membrane Proteins / chemistry Membrane Proteins / metabolism* Microfilament Proteins / chemistry Microfilament Proteins / metabolism* Phorbol Esters / pharmacology Phosphorylation / drug effects Protein Kinase C / antagonists & inhibitors Protein Kinase C / metabolism* Protein Kinase Inhibitors / pharmacology Structure-Activity Relationship
IF 2.985
Times Cited 1
Human and Animal Cells KG-1(RCB1166)