RRC ID 58687
著者 Chu J, Yan B, Zhang J, Peng L, Ao X, Zheng Z, Jiang T, Zhang Z.
タイトル Casticin Attenuates Osteoarthritis-Related Cartilage Degeneration by Inhibiting the ROS-Mediated NF-κB Signaling Pathway in vitro and in vivo.
ジャーナル Inflammation
Abstract Casticin, a flavonoid isolated from Vitex trifolia, has been shown to have anti-inflammatory and antitumor effects in previous studies. Osteoarthritis (OA) is a disease based on degenerative pathological changes. The disease process is often accompanied by inflammatory pathological changes. However, there is no safe and effective drug for prevention and treatment. In the present study, we aimed to clarify the role of casticin in the murine model of destabilization of the medial meniscus (DMM). Male BALB/c mice were randomly divided into three groups: Sham, DMM-induced OA treated with vehicle, and DMM-induced OA treated with casticin. Our results indicated that the casticin treatments markedly reduced the destruction of cartilage and OARSI grades compared with those of the vehicle-treated mice. The levels of matrix metalloproteinase-13 (MMP13) in cartilage were also significantly reduced in the casticin-treated mice. Casticin also significantly regulated oxidative stress and reduced inflammation in the cartilage of mice with OA. These results suggest that casticin prevents the development of posttraumatic OA in mice. Consequently, decreased reactive oxygen species levels and suppressed proinflammatory cytokine production were confirmed in casticin-treated IL-1β-stimulated ADTC5 cells. After casticin treatment, the NF-κB signaling pathway was significantly inhibited in the cells. It can be concluded that casticin can alleviate arthritis-related cartilage degeneration by inhibiting ROS-mediated NF-κB signaling pathway in vitro and in vivo.
巻・号 43(3)
ページ 810-820
公開日 2020-6-1
DOI 10.1007/s10753-019-01167-y
PII 10.1007/s10753-019-01167-y
PMID 31897918
MeSH Animals Cartilage, Articular / drug effects* Cartilage, Articular / metabolism Cartilage, Articular / pathology Cell Line Cell Survival / drug effects Cell Survival / physiology Dose-Response Relationship, Drug Flavonoids / pharmacology Flavonoids / therapeutic use* Male Mice Mice, Inbred C57BL NF-kappa B / antagonists & inhibitors* NF-kappa B / metabolism Osteoarthritis / drug therapy* Osteoarthritis / metabolism Osteoarthritis / pathology Random Allocation Reactive Oxygen Species / antagonists & inhibitors* Reactive Oxygen Species / metabolism Signal Transduction / drug effects Signal Transduction / physiology
IF 3.212
引用数 1
リソース情報
ヒト・動物細胞 ATDC5(RCB0565)