Reference - Detail
| RRC ID | 589 |
|---|---|
| Author | Andersen P, Kragelund BB, Olsen AN, Larsen FH, Chua NH, Poulsen FM, Skriver K. |
| Title | Structure and biochemical function of a prototypical Arabidopsis U-box domain. |
| Journal | J Biol Chem |
| Abstract |
U-box proteins, as well as other proteins involved in regulated protein degradation, are apparently over-represented in Arabidopsis compared with other model eukaryotes. The Arabidopsis protein AtPUB14 contains a typical U-box domain followed by an Armadillo repeat region, a domain organization that is frequently found in plant U-box proteins. In vitro ubiquitination assays demonstrated that AtPUB14 functions as an E3 ubiquitin ligase with specific E2 ubiquitin-conjugating enzymes. The structure of the AtPUB14 U-box domain was determined by NMR spectroscopy. It adopts the betabetaalphabeta fold of the Prp19p U-box and RING finger domains. In these proteins, conserved hydrophobic residues form a putative E2-binding cleft. By contrast, they contain no common polar E2 binding site motif. Two hydrophobic cores stabilize the AtPUB14 U-box fold, and hydrogen bonds and salt bridges interconnect the residues corresponding to zinc ion-coordinating residues in RING domains. Residues from a C-terminal alpha-helix interact with the core domain and contribute to stabilization. The Prp19p U-box lacks a corresponding C-terminal alpha-helix. Chemical shift analysis suggested that aromatic residues exposed at the N terminus and the C-terminal alpha-helix of the AtPUB14 U-box participate in dimerization. Thus, AtPUB14 may form a biologically relevant dimer. This is the first plant U-box structure to be determined, and it provides a model for studies of the many plant U-box proteins and their interactions. Structural insight into these interactions is important, because ubiquitin-dependent protein degradation is a prevalent regulatory mechanism in plants. |
| Volume | 279(38) |
| Pages | 40053-61 |
| Published | 2004-9-17 |
| DOI | 10.1074/jbc.M405057200 |
| PII | S0021-9258(20)72816-4 |
| PMID | 15231834 |
| MeSH | Amino Acid Sequence Arabidopsis / metabolism* Arabidopsis Proteins / chemistry Arabidopsis Proteins / genetics* Arabidopsis Proteins / metabolism* Dimerization Hydrogen Bonding Molecular Sequence Data Protein Structure, Tertiary Ubiquitin / metabolism Ubiquitin-Protein Ligases / chemistry Ubiquitin-Protein Ligases / genetics* Ubiquitin-Protein Ligases / metabolism* Zinc / metabolism |
| IF | 4.238 |
| Times Cited | 60 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 5 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | pda06233 |